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Please note that all translations are automatically generated. Click here for the English version.
200 Views
•
05:06 min
February 2nd, 2024
DOI :
10.3791/200998-v
Transcript
细胞传代前。用冷包衣溶液涂覆六孔板,用5%二氧化碳在37摄氏度下孵育一小时,从含有人多能干细胞的六孔板中吸出干细胞培养基,并在每个孔中加入一毫升无钙镁DPBS。第二次洗涤后,向每个孔中加入 500 微升解离溶液,并在室温下孵育 2 至 5 分钟。
同时,从六孔板中吸出包衣溶液,并在每个孔中加入一毫升无钙镁DPBS。在倒置显微镜下,观察细胞解离过程。当80%至90%的细胞看起来是圆形的和粘附的时,从六孔板的孔中吸出解离溶液。
然后向六孔板中加入一毫升含有10微摩尔岩石
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