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Please note that all translations are automatically generated. Click here for the English version.
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02:17 min
January 19th, 2024
DOI :
10.3791/201044-v
* These authors contributed equally
Transcript
首先,将收集的人类精液样本液化,并使用巴斯德移液管将其彻底均质化。然后将 100 微升精液样品转移到离心管中。向该试管中加入 900 微升染色溶液。
通过移液反复混合并彻底均质化反应物。将管子放在二维振荡器上,在室温下以200RPM连续搅拌30分钟。在等待染色反应的同时,使用CASA系统继续分析精子浓度。
打开系统后,双击SCAscope图标打开应用软件。然后,通过移液将精液样品充分混合,并在 SCA 计数室中转移多达 10 微升的样品。等待 60 秒,让精液样本停
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