The Third Affiliated Hospital of Sun Yat-sen University

6 ARTICLES PUBLISHED IN JoVE

Chemistry

In Situ Characterization of Boehmite Particles in Water Using Liquid SEM

Juan Yao¹, Bruce W. Arey¹, Li Yang¹, Fei Zhang¹, Rachel Komorek¹, Jaehun Chun¹, Xiao-Ying Yu¹

¹Earth & Biological Sciences Directorate, Pacific Northwest National Laboratory

We present a procedure for real-time imaging and elemental composition analysis of boehmite particles in deionized water by in situ liquid Scanning Electron Microscopy.

Developmental Biology

A Mini-Invasive Internal Fixation Technique for Studying Immobilization-Induced Knee Flexion Contracture in Rats

Shihai Jiang^*1, Xiaoyou Yi^*3, Yuansen Luo², Dongjie Yu¹, Yuangao Liu¹, Fei Zhang^1,4, Lei Zhu², Kun Wang¹

¹Department of Joint and Trauma Surgery, The Third Affiliated Hospital of Sun Yat-sen University, ²Department of Plastic Surgery, The Third Affiliated Hospital of Sun Yat-sen University, ³Department of Bone Surgery, Tungwah Hospital of Sun Yat-sen University, ⁴Department of Othopaedic Surgery, The Eighth Affiliated Hospital of Sun Yat-sen University

Here, we present a protocol to describe a minimally invasive technique for knee joint immobilization in a rat model. This reproducible protocol, basing on muscle-gap separation modus and the mini-incision skill, is suitable for studying the underlying molecular mechanism of acquired joint contracture.

Neuroscience

Generation of Human Neurons and Oligodendrocytes from Pluripotent Stem Cells for Modeling Neuron-Oligodendrocyte Interactions

Benedetta Assetta^*1, Changyong Tang^*1,2, Jing Bian^*3, Ryan O'Rourke¹, Kevin Connolly¹, Thomas Brickler³, Sundari Chetty^3,4, Yu-Wen Alvin Huang^1,5,6

¹Department of Molecular Biology, Cell Biology and Biochemistry, Brown University, ²Department of Neurology, The Third Affiliated Hospital of Sun Yat-Sen University, ³Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, ⁴Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine, ⁵Department of Neurology, Warren Alpert Medical School of Brown University, ⁶Center for Translational Neuroscience, Robert J. and Nancy D. Carney Institute for Brain Science and Brown Institute for Translational Science, Brown University

The neuron-glial interactions in neurodegeneration are not well understood due to inadequate tools and methods. Here, we describe optimized protocols to obtain induced neurons, oligodendrocyte precursor cells, and oligodendrocytes from human pluripotent stem cells and provide examples of the values of these methods in understanding cell-type-specific contributions in Alzheimer’s disease.

Medicine

Ameliorating Osteoarthritis in Mice Using Silver Nanoparticles

Yuan Sang^*1, Junbin Zhang^*1, Chang Liu¹, Kaihua Liu¹, Hui Yao¹, Huiqing Zhao¹, Wenbin Xu¹, Yichun Xu¹, Gang Hou¹

¹Department of Orthopedics, The Third Affiliated Hospital of Sun Yat-sen University

Presented here is a protocol for using silver nanoparticles to effectively ameliorate the acute symptoms of type II collagenase-induced osteoarthritis mice, including synovial inflammation, synovial hyperplasia, vascular hyperplasia, etc.

Medicine

The Third Channel-Assisted Unilateral Biportal Endoscopic Technique for Lumbar Spinal Stenosis Combined with Contralateral Disc Herniation

Lixian Tan^1,2,3, Runmin Tang^1,2,3, Limin Rong^1,2,3, Liangming Zhang^1,2,3

¹Department of Spine Surgery, The Third Affiliated Hospital of Sun Yat-sen University, ²Guangdong Provincial Center for Quality Control of Minimally Invasive Spine Surgery, ³Guangdong Provincial Center for Engineering and Technology Research of Minimally Invasive Spine Surgery

Here, we present the third channel-assisted UBE technique, which allows for the vertical removal of herniated disc fragments. This technique can effectively address the limitations of traditional UBE techniques. This article will systematically elaborate on this procedure.

Medicine

The Examination of Peroxidase-Positive Leukocytes in Semen

Xiaoming Li^*1, Xiaohua Cai^*1, Liuhong Cai¹, Jianping Ou¹

¹Center for Reproductive Medicine, The Third Affiliated Hospital of Sun Yat-sen University

This paper presents an economical and efficient protocol for examining peroxidase-positive leukocytes in semen. With the assistance of a computer-assisted semen analysis (CASA) system, the concentration of peroxidase-positive leukocytes in semen can be obtained within a total of 60 min, effectively improving the efficiency of andrology laboratory and andrologists.