Dihydroethidium (DHE) Staining of Hepatocarcinoma Cells for the Quantification of Reactive Oxygen Species (ROS)

To begin, seed 200 microliters volume of 10, 000 hepatocellular carcinoma cells into a 96 well plate with DMEM and 10%FBS. Incubate the cells overnight at 37 degrees Celsius in a humidified incubator under 5%carbon dioxide. The next day, replace t