High-Throughput Sequencing and Bioinformatic Analysis in Grape Must and Wine for Microbial Diversity

To begin, use specific primers 515f and 806r to amplify the V4 hypervariable region of the 16S rNA gene. Perform PCR using the given conditions. Next, add 3.5 microliters of sequencing buffer and 1.5 microliters of enzyme mix to the tube.

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