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Please note that all translations are automatically generated. Click here for the English version.
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02:10 min
December 22nd, 2023
DOI :
10.3791/201242-v
Transcript
首先,在含有Y-27632的维持培养基中解冻并培养H9胚胎干细胞。在第零天,一旦菌落汇合度达到 70%,用 2 毫升 DPBS 洗涤它们,然后用每升含有 20 微摩尔 Y-27632 的 0.5 毫升细胞解离溶液孵育细胞。要中止消化,加入 3 毫升每升含有 20 微摩尔 Y-27632 的维持培养基。
为了沉淀细胞,将试管以190G离心五分钟,然后弃去上清液。将沉淀重悬于一毫升含Y-27632的不含生长因子的化学成分培养基中。细胞计数后,将 120 万个细胞加入 10 毫升不含
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