Medical School of Chinese PLA

3 ARTICLES PUBLISHED IN JoVE

Medicine

A Model of Long-Term Ventricular Fibrillation in Isolated Rat Hearts

Xiaoyi He^1,2, Libing Li¹, Wenhong Xu¹, Shengli Jiang^1,2

¹Cardiovascular Surgery, First Medical Center, Chinese PLA General Hospital, ²Medical School of Chinese PLA

This protocol presents a model of long-term ventricular fibrillation in rat hearts induced by continuous stimulation with low-voltage alternating current. This model has a high success rate, is stable, reliable, and reproducible, has a low impact on cardiac function, and causes only mild myocardial injury.

Developmental Biology

Generating Neural Retina from Human Pluripotent Stem Cells

Wen Li^*1,2, Hongyu Li^*3, Hao Yan³, Lixiong Gao², Xiaoling Wang³, Lingping Zhao³, Yuhan Yan^1,2, Zi Ye², Jiafei Xi³, Wen Yue³, Zhaohui Li^1,2

¹Medical School of Chinese PLA, ²Senior Department of Ophthalmology, The Third Medical Center of Chinese PLA General Hospital, ³Stem Cell and Regenerative Medicine Lab, Beijing Institute of Radiation Medicine

The present protocol describes an optimized 3D neural retina induction system that reduces the adhesion and fusion of retinal organoids with high repeatability and efficiency.

Medicine

Technique for Isolation and Culture of Rat Jaw Bone Marrow Mesenchymal Stem Cells

Tianqi Li^1,2,3, Xiangbo Meng^1,2, Shuaichen Li^1,2, Sunxin Zhou^1,2, Hongkun Li^1,2, Shi Quan², Tong Zhang²

¹Medical School of Chinese PLA, ²Department of Stomatology, the First Medical Centre, Chinese PLA General Hospital, ³Department of Stomatology, Fujian People's Armed Police Corps Hospital

Mesenchymal stem cells from the jaw bone marrow have significant functions in diverse differentiation, self-renewal, and immune modulation. They have emerged as a crucial reservoir of precursor cells in gene therapy, tissue engineering, and regenerative medicine. Here, we present a unique method for isolating jaw bone marrow mesenchymal stem cells in rats.