Murine Neonatal Brain Sample Fixation and Staining for Micro-CT Scanning

To begin, fill the 50 milliliter conical tube with 40 milliliters of 4%paraformaldehyde. And immerse freshly decapitated neonatal heads into it using a paton spatula or a spoon. Store the tubes at minus four degrees celsius for 48 hours.

Then transfer the heads to 40 milliliters of PBS containing 0.1%sodium azide, and continue to keep the heads refrigerated. For staining, immerse each head in a 50 milliliter conical tube containing 40 milliliters of Logul's solution, and mix it for 15 to 20 hours on an orbital shaker at a constant stir. Before scanning, place the stained sample in a 15 milliliter tube containing 10 milliliters of 1%agros for 30 minutes.