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Please note that all translations are automatically generated. Click here for the English version.
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01:05 min
March 8th, 2024
DOI :
10.3791/201359-v
Transcript
首先,设置脑切片恢复和维护工作区。准备脑切片准备工作区并设置振动切片机。接下来,设置大脑提取和准备工作区。
然后将提取的小鼠大脑转移到大脑制备工作区中带有冷却碳酸切割溶液的 90 毫米塑料培养皿中。定向大脑以在琼脂糖模具中定位。将强力胶涂在琼脂糖模具的喙端。
用刮刀将大脑放在模具中,背侧朝下进行水平切片。切片海马体和嗅球组织。并使用玻璃巴斯德移液管,在每轮后收集切片。
将切片在充满人工脑脊液的恢复室中在32摄氏度水浴中孵育45分钟,然后在室温下孵育一小时。
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