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Please note that all translations are automatically generated. Click here for the English version.
33 Views
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01:31 min
January 26th, 2024
DOI :
10.3791/201366-v
* These authors contributed equally
Transcript
首先,将安乐死的小鼠放在手术平台上并打开胸腔。使用手术剪刀,在肋骨下方做一个横向切口,然后切开小鼠两侧到锁骨的肋骨。然后切开横膈膜以释放胸壁,并用镊子提起肋骨以暴露胸腔。
用镊子握住胸腺,轻轻切开将胸腺固定到位的结缔组织。为了进行小鼠胸腺组织的酶解离,将清洁的胸腺放入15毫升的管帽中,并使用无菌手术剪刀将组织细碎。向试管中加入两毫升小鼠解离混合物。
连接包含胸腺片的盖子,并将管子倒置五次以重新悬浮组织。用石蜡膜包裹管帽。孵育后,将试管放在架子上,让组织沉降。
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