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Please note that all translations are automatically generated. Click here for the English version.
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03:49 min
March 8th, 2024
DOI :
10.3791/201404-v
Transcript
首先,在1.5毫升微量离心管中制备GUV外部缓冲溶液。将亚精胺,ATP,GTP,CTP,UTP,磷酸肌酸,醋酸镁,谷氨酸钾,HEPES,氢氧化钾,亚叶酸混合在一起。葡萄糖和氨基酸储备液。
加入超纯去离子水,使溶液的最终体积达到一毫升。接下来,在通风橱中,将 17.3 微升 POPC 储备溶液加入 15 毫升玻璃瓶中。为此,移液1.08微升PEO-b-PBD共聚物。
小心地将一股温和的氩气吹入玻璃瓶中,同时旋转小瓶以蒸发氯仿。然后将 1.2 毫升轻质矿物油移液到玻璃瓶
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