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Please note that all translations are automatically generated. Click here for the English version.
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02:18 min
March 29th, 2024
DOI :
10.3791/201428-v
* These authors contributed equally
Transcript
要开始培养光滑念珠菌,请将所有实验材料放在工作平台上。从酵母蛋白胨葡萄糖或 YPD 琼脂平板中挑选单个 C.glabrata 菌落。并将其转移到含有 3 毫升 YPD 培养基的玻璃试管中。
在 25 摄氏度下孵育 14 至 16 小时,以 155 RPM 的速度振荡。孵育后,在新鲜 YPD 中稀释培养物,以获得 600 纳米的光密度为 0.1。将稀释的培养物在 25 摄氏度下孵育 6 小时,以 155 RPM 振荡。
制备酵母悬浮液,光密度为600纳米,为0.1,在
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