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Please note that all translations are automatically generated. Click here for the English version.
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01:47 min
March 29th, 2024
DOI :
10.3791/201429-v
* These authors contributed equally
Transcript
首先,将光滑念珠菌培养物和 YPD 培养基放在工作平台上。用YPD培养基将600纳米的酵母细胞悬浮液的光密度调节至0.1。将 1 毫升细胞悬液转移到 5 毫升管中。
将无菌棉签浸入酵母细胞悬浮液中。在Mueller Hinton琼脂板上来回擦拭棉花,以60度旋转两次。然后擦拭周边以均匀覆盖。
使用记号笔将板分成三个相等的扇区。将镊子放在火焰上消毒一到两秒钟。冷却后,将一个空白磁盘放在每个扇区的中心。
向每个光盘中加入 12.5 微升氟康唑原液。充分混合以
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