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Please note that all translations are automatically generated. Click here for the English version.
20 Views
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01:57 min
March 1st, 2024
DOI :
10.3791/201454-v
Transcript
首先,将 30 微升刀豆球蛋白 A 珠加入 1.5 毫升离心管中。吸取 300 微升结合缓冲液到珠子上。将试管多次倒置以充分混合,然后将试管放在磁性架上。
溶液澄清后,用移液管吸出上清液。从架子上取出后,将 300 微升结合缓冲液移液到试管中。再次倒置以混合管中的内容物。
将试管的内容物均匀地分布到三个管中,每个管子有八个PCR管条,然后将管条放在磁性架上,直到溶液澄清。除去上清液后,将 10 微升结合缓冲液移液到试管中并混合。将准备好的珠子放在冰上,直到进一步实验
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