Using Cleavage Under Targets and Tagmentation (CUT&Tag) Assay in Mouse Myoblast Research

573 Views

•

07:09 min

•

March 1st, 2024

DOI :

10.3791/66066-v

March 1st, 2024

•

Yuefeng Li¹^,², Xiaofen Wu¹^,², Ping Hu¹^,²

¹Guangzhou Laboratory, ²The Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University

Transcript

Summary

Explore More Videos

CUT Tag

Genome Structure

Transcription

Skeletal Muscle Stem Cells

Chromatin Immunoprecipitation

Rare Cell Populations

Chapters in this video

0:00

Introduction

1:15

Sequestration of Mouse Myoblasts onto Activated Concanavalin‐A Beads

3:08

Incubation of Mouse Myoblast‐Sequestered Concanavalin‐A Beads with Antibodies for CUT&Tag Assay

4:59

Genomic Purification and Library Preparation of Tagmented Mouse Myoblast DNA for Epigenetic Profiling

Related Videos

10:39

Contrast Imaging in Mouse Embryos Using High-frequency Ultrasound

15.3K Views

10:56

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

21.1K Views

08:56

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

8.2K Views

06:29

Salinity-dependent Toxicity Assay of Silver Nanocolloids Using Medaka Eggs

7.9K Views

10:48

In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity

8.2K Views

09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

12.6K Views

09:36

Visualizing the Node and Notochordal Plate In Gastrulating Mouse Embryos Using Scanning Electron Microscopy and Whole Mount Immunofluorescence

8.7K Views

09:24

Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

9.7K Views

11:02

Establishing Organoids from Human Tooth as a Powerful Tool Toward Mechanistic Research and Regenerative Therapy

3.2K Views

10:10

An Efficient Protocol for CUT&RUN Analysis of FACS-Isolated Mouse Satellite Cells

2.1K Views