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Please note that all translations are automatically generated. Click here for the English version.
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02:46 min
February 16th, 2024
DOI :
10.3791/201539-v
Transcript
首先,对人子宫成纤维细胞进行 70% 至 90% 的融合培养。从烧瓶中吸出培养基。用 5 毫升温热的不含钙和镁的 DPBS 清洗细胞。
吸出 DPBS 后,将 4 毫升温热的胰蛋白酶 EDTA 移液到每个烧瓶中。将细胞在 37 摄氏度下孵育 3 到 5 分钟,直到细胞分离。接下来,向每个烧瓶中加入 6 毫升滴注和中和溶液。
然后将细胞悬液转移到 15 毫升锥形管中。使用移液管将悬浮液充分混合,并取 10 微升等分试样进行细胞计数。将 10 微升细胞悬液与 10 微升
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