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Please note that all translations are automatically generated. Click here for the English version.
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02:17 min
February 16th, 2024
DOI :
10.3791/201540-v
Transcript
首先,将 50 毫升(成纤维细胞培养基和阴道上皮培养基)各取 50 毫升,放入单独的 50 毫升锥形管中。在无菌真空下对加热的培养基进行脱气五分钟。接下来,用 CaviCide 和乙醇对动态流量模块的清洁托盘进行消毒。
从包装中取出 pod 并将它们放入动态流量模块托盘中。将 2 毫升脱气的阴道上皮培养基移液到顶端入口储液器中。并向基底入口储液罐中加入 3 毫升脱气成纤维细胞培养基。
将 500 微升脱气阴道上皮培养基移液至根尖出口储液器,将 500 微升脱气成纤维
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