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Please note that all translations are automatically generated. Click here for the English version.
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02:00 min
March 8th, 2024
DOI :
10.3791/201543-v
Transcript
首先,将 80 微升转化的大肠杆菌细胞移液到小瓶中。将 20 微升 4 倍 Laemmli SDS 上样缓冲液移入小瓶中。将悬浮液在加热块中以 100 摄氏度煮沸 10 分钟。
然后将 10 μL 样品加载到预制 SDS 凝胶上。在室温下将凝胶在SDS电泳缓冲液中以120伏电泳一小时。电泳完成后,在考马斯染色剂中染色凝胶一小时。
在含有50%甲醇和10%乙酸的脱色缓冲液中,在蒸馏水中对凝胶进行脱色两小时。将凝胶放入凝胶成像系统中以可视化蛋白质条带。接下来,将SDS凝
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