To begin, position the oophorectomized menopause model rat on a non slippery mat and locate the acupoints for the treatment. Using a pet electric clipper, shave the acupuncture sites and mark them with an oil-based marker. With the left hand, hold the rat's tail and gently pull back.
Open the port of the rat coat with the right hand, and carefully let the rat burrow into it. When the rat reaches the middle of the coat, hold both sides of the rat's shoulder blades with the right hand and pull out the rat's forelimbs with the left hand. After tightening the ties on the front of the rat coat, position the hind limbs of the rat outside the coat.
For the swing type suspension, fix two S hooks into the knot on the back of the rat coat and hang them on the iron frame to suspend the rat. Sterilize the local area of the rat three times with 5%ionophore, followed by 75%alcohol. Use hemostatic forceps to hold a cotton ball soaked in 95%alcohol and light it with a lighter.
Then, with the right hand, hold the acupuncture needle between the thumb, forefinger, and middle finger. Hold both the needle tip and the body of the needle onto the flaming cotton ball until it turns red. Next, holding the needle vertically above the acupoint, quickly puncture the point and pull out within one second.
Finally, press a sterile dry cotton ball to the needle hole to prevent bleeding. Compared with the normal group, a large number of trabeculae were lost in the femur cancellous matrix in the model group. However, there was a large increase in the thickness of trabeculae in the center and periphery of the cancellous bone of the femur in the fire needle group, as compared to the model group.
