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Please note that all translations are automatically generated. Click here for the English version.
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01:59 min
May 31st, 2024
DOI :
10.3791/201727-v
Transcript
首先,洗涤 RNA-模板 C-star 缩合物,让提取的缩合物溶液沉淀至少 5 分钟。然后,从液位顶部移液以尽量减少冷凝物去除,提取大约一半体积的上清液。在 tris-EDTA 中加入相同体积的 0.3 摩尔氯化钠以替换去除的上清液,并通过移液混合。
重复上清液去除和缓冲液更换的循环,总共三个循环。对于 T7 转录混合物,制备 10 毫摩尔 DFHBI 的二甲基亚砜储备液。然后使用不含 RNAse 和 DNAse 的水将等分试样稀释至终浓度为 600 μmol。
在
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