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Please note that all translations are automatically generated. Click here for the English version.
16 Views
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02:46 min
May 3rd, 2024
DOI :
10.3791/201731-v
Transcript
首先,将 10 微升 2X 肽溶液液滴移到 24 孔板的孔中心。孵育后,向每个孔中移取 10 μL 2X PBS。使用移液器的尖端轻轻混合溶液,让凝胶液滴稳定至少 20 分钟,确保液滴完好无损。
接下来,将 2.5 毫升 0.0125% 胰蛋白酶 EDTA 移液到含有 200 万个 SW1222 人结直肠腺癌细胞的培养瓶中。将细胞与胰蛋白酶在 37 摄氏度下孵育 5 到 10 分钟,直到它们从培养瓶中分离。加入 5 毫升完全培养基以灭活胰蛋白酶。
然后使用 30 微
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