Assessing Cell Viability of Caco-2 Cells Treated with Pesticide Metabolite Extracts from Pesticide-Treated Carrot Callus

To begin, retrieve the 96-well plate containing the cells from the incubator after allowing a 24-hour exposure to the metabolite extract solutions. Dispose of the culture medium and perform a double wash of the cells using PBS. Introduce 100 microliters of DMEM into each well, followed by the addition of 10 microliters of CCK-8 reagents.

Ensure even distribution of the cells by gently shaking the plate. Incubate the cells at 37 degrees Celsius in 5%carbon dioxide for four hours. Finally, conduct an optical density measurement at 450 nanometers using a fluorescence spectrophotometer.

No significant difference was found between the viability of the cells in the pesticide metabolite and control group, indicating no cytotoxicity of pesticide metabolites. The cell viability in the pesticide parent group was significantly lower as compared to the control, indicating obvious cytotoxicity.