Fluorescence Assay for the Detection of Iron Chelators Using Calcein and Ferrous Ions

To begin, use a multi-channel pipette to distribute 50 microliters of PBS into columns 2 to 10 of rows A to E on a 96-well plate. Pipette 100 microliters of two millimolar ferric ammonium sulfate stock solution into column 11. With a multi-channel