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01:33 min
June 28th, 2024
DOI :
10.3791/202012-v
* These authors contributed equally
Transcript
首先,使用手术刀解剖 4% 多聚甲醛固定的人体心脏组织,使其适合共聚焦显微镜检查室。将分级甲醇系列中的心脏标本在室温下搅拌脱水 1 小时,然后在 66% 二氯甲烷和 33% 甲醇溶液中搅拌过夜。第二天,通过执行指示的步骤漂白样品。
然后用分级甲醇系列再水化样品 1 小时,每次搅拌。免疫标记后,请执行以下步骤准备组织进行成像。将含有粘合剂的腔室固定在载玻片上,并在腔室周边涂抹指甲油。
打开配备 5X
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