Creating a Self-Sealing Corneal Tunnel for Intracameral Injection in Rats

To begin, prepare the animal for surgery and apply 0.3%ofloxacin and 0.1%dexamethasone to the animal's eye. Using surgical ophthalmic forceps, hold the superior sclera at the vertical midline next to the corneoscleral junction to stabilize the eye. Under a surgical microscope, place a sterile 0.8 millimeter 31-gauge stiletto blade in the paracentral corneal region on the vertical midline.

In this position, puncture the cornea to make an incision and create a long tunnel until it penetrates into the central area of the anterior chamber. Then apply topical 0.3%ofloxacin and 0.1%dexamethasone to the eye. Next, under a slit microscope, observe the depth of the anterior chamber of the injected eye compared to the non-injected eye.

Also observe the lens of the injected eye compared to the non-injected eye.