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02:18 min
July 12th, 2024
DOI :
10.3791/202080-v
Transcript
首先,取培养的小鼠卵巢上皮癌细胞。从培养皿中取出培养基,用 DPBS 洗涤两次。加入 20 mL 不含 FBS 的 Dulbecco 改良 Eagle 培养基和青霉素-链霉素溶液。
在 37 摄氏度和 5% 二氧化碳下孵育 48 小时。然后从 20 个直径为 15 厘米的培养皿中收集细胞培养上清液。在 4 摄氏度下以 300 G 离心 10 分钟,然后收集上清液。
将上清液在 2, 000 G 下在
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