Name: orthotopic hind-limb transplantation in rats
Uploaded: 2010-07-12T17:40:00
Description: Watch this Scientific Journal Video about Orthotopic Hind-Limb Transplantation in Rats at JoVE.com

<p class="jove_title">捐助者的操作和后肢收成</p><ol><li>对于执行过程中的显微仪器的使用无菌集。外科医生应穿着合适的服装，包括无菌手套，要改变的手术开始前。 </li><li>大鼠腹腔注射戊巴比妥（50mg/kg的IP）麻醉，仰卧放在加热的经营领域，这有利于整个手术过程中的一个稳定的动物体温。 </li><li>皮肤切口前，提交操作是消毒三次，每个都，消毒剂和酒精。为了防止动物眼角膜干燥，眼睛眼药膏（Vidisic）手术前的...

<ol>
	<li>Shapiro, R. I., Cerra, F. B. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+model+for+reimplantation+and+transplantation+of+a+complex+organ:+the+rat+hind+limb.">A model for reimplantation and transplantation of a complex organ: the rat hind limb.</a> <em style='font-style: italic'>J Surg Res</em>. <b>24</b> (6), 501-506 (1978).</li><li>Petruzzo, P. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+international+registry+on+hand+and+composite+tissue+transplantation.">The international registry on hand and composite tissue transplantation.</a> <em style='font-style: italic'>Transplantation</em>. <b>86</b> (4), 487-492 (2008).</li><li>Schneeberger, S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=First+forearm+transplantation:+outcome+at+3+years.">First forearm transplantation: outcome at 3 years.</a> <em style='font-style: italic'>Am J Transplant</em>. <b>7</b> (7), 1753-1762 (2007).</li></ol>

<p class="jove_content">数十年的实验研究之前，启用成功的人的手移植在最近的过去。 1978年，夏皮罗等。开发后肢大鼠原位移植模型，非常适合于研究各种病理，生理和免疫学方面以及功能的复合组织移植的结果。我们已经修改了程序的最具挑战性的的部分，即血管吻合，通过引入非缝合袖口技术。相比传统的缝合技术，它简化和缩短手术过程，使基本的显微手术经验的外科医生进行，成活率高（80-90％）的程序。 </...

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orthotopic hind-limb transplantation in rats

<p class="jove_content">复合组织异体移植（CTA）后失去一只手，前臂或数字代表了有效的治疗选择，并已成为一种新型的整形外科治疗实体。然而，长期高剂量的多种药物的免疫抑制治疗是必需的，以确保移植物的存活，轴承的严重副作用笼头更广泛的应用的风险。在这一领域的进一步恶化可能依赖于更好地理解基础免疫学和复合组织移植缺血再灌注损伤。 </p><p class="jove_content">迄今为止，原位移植在大鼠后肢已啮齿动物模型重建移植（RT）的首选，但是，它是一个非常艰巨的过程，需要非凡复位血管，骨骼，肌肉和神经的显微外科技能。 </p><p class="jove_content">我们引进了这种模式的血管袖口吻合技术，提供快速和可靠的的方法，以大鼠后肢移植。这项技术简化和缩短手术过程，使基本的显微手术经验的外科医生成功地执行操作，成活率高和低并发症发生率。这项技术似乎能够很好地适用于免疫学以及缺血再灌注损伤（IRI）的研究。 </p>

Watch this Scientific Journal Video about Orthotopic Hind-Limb Transplantation in Rats at JoVE.com

Orthotopic Hind-Limb Transplantation in Rats

<p class="jove_content">在这里，我们描述的原位大鼠后肢移植过程中，这似乎是黄金标准<em&gt;在体内</em&gt;复合组织异体移植研究模型。</p>

原位大鼠后肢移植

Composite tissue allotransplantation (CTA) now represents a valid therapeutic option after the loss of a hand, forearm or digits and has become a novel ...

Research

JoVE Journal

Immunology and Infection

Diagnosis of Ecto- and Endoparasites in Laboratory Rats and Mice

Ecto和实验室大鼠和小鼠体内寄生虫的诊断

Internal and external parasites remain a significant concern in laboratory rodent facilities, and many research facilities harbor some parasitized ...

科研

免疫与感染

<em>In vivo</em> Imaging Method to Distinguish Acute and Chronic Inflammation

In vivo Imaging Method to Distinguish Acute and Chronic Inflammation

<em>在体内</em>成像方法区分急性和慢性炎症

Inflammation is a fundamental aspect of many human diseases. In this video report, we demonstrate non-invasive bioluminescence imaging techniques that ...

Long Term Chronic <em>Pseudomonas aeruginosa</em> Airway Infection in Mice

Long Term Chronic Pseudomonas aeruginosa Airway Infection in Mice

长期慢性<em&gt;铜绿假单胞菌</em&gt;在呼吸道感染小鼠

A mouse model of chronic airway infection is a key asset in cystic fibrosis (CF) research, although there are a number of concerns regarding the model ...

Complete Thymectomy in Adult Rats with Non-invasive Endotracheal Intubation

完整的胸腺切除术的成年大鼠非侵入气管插管

Thymectomy in neonatal rodents is an established and reliable procedure for immunological studies. However, in adult rats, complications of hemorrhage and ...

Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation

鼠后肢长骨解剖和骨髓隔离

Investigation of the bone and the bone marrow is critical in many research fields including basic bone biology, immunology, hematology, cancer metastasis, ...

Murine Full-thickness Skin Transplantation

鼠全层皮肤移植

Murine full-thickness skin transplantation is a well-established in vivo model to study alloimmune response and graft rejection. Despite its limited ...

<em>In Vitro</em> and <em>In Vivo</em> Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

<em>体外</em>和<em>活体</em>评估移植受体的 T、B 和髓细胞抑制活性和体液反应

The main concern in transplantation is to achieve specific tolerance through induction of regulatory cells. The understanding of tolerance mechanisms ...

An Immunological Model for Heterotopic Heart and Cardiac Muscle Cell Transplantation in Rats

大鼠异位心脏和心肌细胞移植的免疫模型

Heterotopic heart transplantation in rats has been a commonly used model for diverse immunological studies for more than 50 years. Several modifications ...

Optimization of the Cuff Technique for Murine Heart Transplantation

穆林心脏移植袖口技术的优化

Murine cardiac transplantation has been performed for more than 40 years. With advancements in microsurgery, certain new techniques have been used to ...

Bone Marrow Transplantation Procedures in Mice to Study Clonal Hematopoiesis

小鼠骨髓移植程序研究克隆造血症

Clonal hematopoiesis is a prevalent age-associated condition that results from the accumulation of somatic mutations in hematopoietic stem and progenitor ...