Graft Preparation for Loading in Ex Vivo Normothermic Machine Perfusion Systems

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00:57 min

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August 2nd, 2024

DOI :

10.3791/202224-v

August 2nd, 2024

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George Olverson IV*¹, Manuela Lopera Higuita*², Maya Bolger-Chen², Emmanuella O. Ajenu², Selena S. Li¹, Hussein Kharroubi¹, Bassel Tfayli¹, Chijioke Chukwudi¹, Nathan Minie¹, Joseph Catricala¹, Allison Pitti¹, William Michaud¹, Doug Vincent³, David D’Alessandro¹, S. Alireza Rabi¹, Shannon N. Tessier², Asishana A. Osho¹

¹Division of Cardiac Surgery, Massachusetts General Hospital, Harvard Medical School, ²Center for Engineering in Medicine and Surgery, Massachusetts General Hospital and Harvard Medical School, Shriners Children's Boston, ³VentriFlo Inc

* These authors contributed equally

Transcript

To begin, take the isolated pig's heart and cut the branches of the aortic arch to form a single outflow tract. Insert the aortic cannula through this tract and secure it using a zip tie and 4-0 silk suture. Then place a bipolar pacing wire on the posterior wall of the right ventricle.

Create two per string 4-0 prolene sutures around the perimeter of the left atrium tract. Secure the sutures with tourniquet snares, but do not tie them until loading. Lastly, close the left atrium appendage with a simple continuous 4-0 prolene suture.

Then record the initial heart weight.

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