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DOI :
10.3791/2039-v
•
11:14 min
July 26th, 2010
Chapters
0:04
Title
0:12
Introduction
1:39
Preparation of PF Library-DNA Fragments
3:26
Preparation of S100B-bound Beads
4:55
Selection & Recovery of Aptamers
6:40
TOPO Cloning and Sequencing
7:17
Sandwich Binding Assays
9:11
Representative Results
10:35
Conclusion
SELEX协议包括多轮选拔,其中每一个需要结合的配体,这反过来又需要固定的引物序列,侧翼的随机库地区的再生。这些固定的引物序列,可以干扰与选择过程(误报和底片)。在这里,我们目前无底漆的协议。
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