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DOI :
10.3791/2045-v
•
12:58 min
October 24th, 2010
Chapters
0:00
Title
1:03
Introduction
1:39
Preparation of Bacterial ( N. meningitidis) Suspension
3:09
Seeding and Infection of Target Cells
4:29
Immuno-staining
6:21
Confocal Laser Scanning Microscopy
8:56
Quantitative Colocalization Analysis
9:55
Intracellular Localization of Opc-expressing N. meningitidis and Alpha-actinin
12:23
Conclusion
脑膜炎奈瑟菌 (NM),革兰氏阴性人类特有的呼吸道病原体,人类α-辅肌动蛋白结合。在这里,我们提出了一个可视化的细菌colocalisation后细菌进入到人脑微血管内皮细胞(HBMECs)的α-辅肌动蛋白与细胞内的协议。
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