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DOI :
10.3791/2045-v
•
12:58 min
October 24th, 2010
Chapters
0:00
Title
1:03
Introduction
1:39
Preparation of Bacterial ( N. meningitidis) Suspension
3:09
Seeding and Infection of Target Cells
4:29
Immuno-staining
6:21
Confocal Laser Scanning Microscopy
8:56
Quantitative Colocalization Analysis
9:55
Intracellular Localization of Opc-expressing N. meningitidis and Alpha-actinin
12:23
Conclusion
髄膜炎菌 (ナノメートル)、グラム陰性ヒト特異的な呼吸器病原体は、ヒトα-アクチニンに結合することができる。ここでは、人間の脳の微小血管内皮細胞(HBMECs)への細菌侵入後のα-アクチニン細胞と細菌のcolocalisationの可視化のためのプロトコルを提示する。
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