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JoVE Journal

Neuroscience

This content is Open Access.

Ultrasound-Guided Microinjection into the Mouse Forebrain In Utero at E9.5

17.8K Views

07:47 min

November 13th, 2010

November 13th, 2010

17,756 Views

0:00

Title

1:56

Exposure of the Embryos

3:45

Loading Virus into a Microinjection Needle

4:49

Ultrasound-guided Viral Injection

6:25

Representative Results

7:05

Conclusion

In utero survival surgery in mice permits the molecular manipulation of gene expression during development. Here we describe the use of high-frequency ultrasound imaging to guide the injection of retroviral vectors into the mouse brain at embryonic day (E) 9.5.

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