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DOI :
10.3791/2055-v
•
9:03 min
December 22nd, 2010
Chapters
0:05
Title
1:02
Introduction
1:32
Dechorionating Medaka Eggs
4:22
Cell Transplantation in Medaka Embryos
6:05
Mounting Dechorionated Embryos
7:35
Images of Post-Transplantation Embryos
8:31
Conclusion
由于硬的蛋壳和软的胚胎,操纵鳉胚胎是比在斑马鱼中更多地参与。该视频显示一步一步的程序,包括dechorionation如何操作鳉胚胎,在琼脂糖凝胶成像和细胞移植嵌合体的生产安装。这些程序是必不可少的,在实验室中使用鳉和斑马鱼为脊椎动物的基因组功能基因清扫充分利用其互补性的特点。
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