Name: chronic imaging of mouse visual cortex using a thinned-skull preparation
Uploaded: 2010-10-25T17:00:00
Description: Watch this Scientific Journal Video about Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation at JoVE.com

Questo lavoro è stato sostenuto dal Career Award Burroughs Wellcome nel Scienze Biomediche (AKM), The Whitehall Research Foundation Grant (AKM), Sloan Foundation Fellowship (AKM), NIH EY019277 (AKM), e un NEI finanziato, Vision Training Grant, EY013319 ( EAK).

<ol><li> Per visualizzare i neuroni nel cervello intatto utilizzando la microscopia a due fotoni, una preparazione in cui i neuroni sono etichettati con marcatori fluorescenti viene utilizzato. Negli esperimenti qui presentati usiamo la GFP-M linea di topi transgenici che le etichette strato di 5 cellule piramidali 1. Layer 5 cellule piramidali progetto processi dendritiche in strati superficiali, permettendo la visualizzazione dei dendriti e spine dendritiche fino ad una profondità di 300 micron al di sotto del livello della pia. U...

<ol>
	<li>Feng, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+neuronal+subsets+in+transgenic+mice+expressing+multiple+spectral+variants+of+GFP.">Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP.</a> Neuron. 28 (1), 41-51 (2000).</li><li>Holtmaat, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=high-resolution+imaging+in+the+mouse+neocortex+through+a+chronic+cranial+window.">high-resolution imaging in the mouse neocortex through a chronic cranial window.</a> Nat Protoc. 4 (8), 1128-1144 (2009).</li><li>Majewska, A. K., Newton, J. R., Sur, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Remodeling+of+synaptic+structure+in+sensory+cortical+areas+in+vivo.">Remodeling of synaptic structure in sensory cortical areas in vivo.</a> J Neurosci. 26 (11), 3021-3029 (2006).</li><li>Mostany, R., Portera-Cailliau, C. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+Craniotomy+Surgery+Procedure+for+Chronic+Brain+Imaging.">A Craniotomy Surgery Procedure for Chronic Brain Imaging.</a> J Vis Exp. , (2008).</li><li>Yang, G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Thinned-skull+cranial+window+technique+for+long-term+imaging+of+the+cortex+in+live+mice.">Thinned-skull cranial window technique for long-term imaging of the cortex in live mice.</a> Nat Protocols. 5 (2), 201-208 (2010).</li><li>Xu, H. T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Choice+of+cranial+window+type+for+in+vivo+imaging+affects+dendritic+spine+turnover+in+the+cortex.">Choice of cranial window type for in vivo imaging affects dendritic spine turnover in the cortex.</a> Nat Neurosci. 10 (5), 549-551 (2007).</li></ol>

Immagini cronico usando la microscopia a due fotoni sta diventando una tecnica sempre più popolare per studiare i cambiamenti morfologici sono innescate durante 2-5 plasticità. Qui dimostrare un assottigliato-teschio preparazione da seguire identificato spine dendritiche nel cervello di topo intatto nei giorni di imaging diverse. In questo protocollo, il cranio è lasciato intatto, causando danni minimi alla corteccia e conseguente livelli molto bassi di neuroinfiammazione che possono alterare la funzione d...

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<td>TC-1000 Temp. Control System for Mice</td>
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chronic imaging of mouse visual cortex using a thinned-skull preparation

Imaging in vivo con due fotoni microscopia a scansione laser (2PLSM) permette lo studio delle cellule viventi e dei processi neuronali nel cervello intatto. La tecnica qui presentata permette l&#39;imaging della stessa area del cervello, in momenti diversi (cronica imaging) con risoluzione microscopica che permette il tracciamento delle spine dendritiche, che sono le strutture di piccole dimensioni che rappresentano la maggioranza dei siti post-sinaptici eccitatori nel SNC. La capacità di risolvere in modo chiaro multa strutture corticali sui punti più volte ha molti vantaggi, in particolare nello studio della plasticità del cervello in cui i cambiamenti morfologici a sinapsi e rimodellamento del circuito può contribuire a spiegare i meccanismi sottostanti. In questo materiale video e complementari, mostriamo un protocollo per la cronica imaging in vivo del cervello intatto con un assottigliato-teschio di preparazione. Il diradamento-cranio preparazione è un approccio mini-invasivo che evita potenziali danni per la durata e / o corteccia, riducendo così l&#39;insorgenza di una risposta infiammatoria. Quando questo protocollo è eseguita correttamente, è possibile monitorare in modo chiaro i cambiamenti nelle caratteristiche spine dendritiche nel cervello intatto per un periodo prolungato di tempo.

Watch this Scientific Journal Video about Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation at JoVE.com

Chronic Imaging of Mouse Visual Cortex Using a Thinned-skull Preparation

In questo materiale video e supplementare, mostriamo un protocollo per la cronica<em&gt; In vivo</emL&#39;imaging&gt; del cervello intatto con un assottigliato-teschio di preparazione.

Imaging cronica di mouse corteccia visiva Utilizzando un Assottigliato-teschio di preparazione

In vivo imaging using two-photon laser scanning microscopy (2PLSM) allows the study of living cells and neuronal processes in the intact brain. The ...

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The field of mouse phenotyping with magnetic resonance imaging (MRI) is rapidly growing, motivated by the need for improved tools for characterizing and ...

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Preparazione di Synaptoneurosomes da mouse Cortex con un discontinuo di Percoll-Saccarosio gradiente di densità

Synaptoneurosomes (SNs) are obtained after homogenization and fractionation of mouse brain cortex. They are resealed vesicles or isolated terminals that ...

A Polished and Reinforced Thinned-skull Window for Long-term Imaging of the Mouse Brain

Un lucido e rinforzato Assottigliato cranio-Window a lungo termine Imaging del cervello di topo

In vivo imaging of cortical function requires optical access to the brain without disruption of the intracranial environment. We present a method to form ...

Thinned-skull Cortical Window Technique for In Vivo Optical Coherence Tomography Imaging

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Optical coherence tomography (OCT) is a biomedical imaging technique  with high spatial-temporal resolution. With its minimally invasive approach OCT  has ...

Functional Imaging of Auditory Cortex in Adult Cats using High-field fMRI

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Current knowledge of sensory processing in the mammalian auditory system is mainly derived from electrophysiological studies in a variety of animal ...

Live Imaging of Mitosis in the Developing Mouse Embryonic Cortex

Immagini dal vivo della mitosi in via di sviluppo embrionali di topo Cortex

Although of short duration, mitosis is a complex and dynamic multi-step process fundamental for development of organs including the brain. In the ...

Two-Photon in vivo Imaging of Dendritic Spines in the Mouse Cortex Using a Thinned-skull Preparation

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Two-Photon<em&gt; In vivo</em&gt; Imaging di spine dendritiche nella corteccia mouse Uso un teschio Diluito-Preparazione

In the mammalian cortex, neurons form extremely complicated networks and exchange information at synapses. Changes in synaptic strength, as well as ...

Measuring Connectivity in the Primary Visual Pathway in Human Albinism Using Diffusion Tensor Imaging and Tractography

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In albinism, the number of ipsilaterally projecting retinal ganglion cells (RGCs) is significantly reduced. The retina and optic chiasm have been proposed ...

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During cerebral cortex development, progenitor cells undergo several rounds of symmetric and asymmetric cell divisions to generate new progenitors or ...

Neurovascular Network Explorer 2.0: A Simple Tool for Exploring and Sharing a Database of Optogenetically-evoked Vasomotion in Mouse Cortex In Vivo

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The importance of sharing experimental data in neuroscience grows with the amount and complexity of data acquired and various techniques used to obtain ...