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DOI :
10.3791/2063-v
June 28th, 2010
Chapters
0:00
Title
0:05
Introduction
1:42
Tissue Preparation
2:29
Immunofluorescence Staining
5:19
Microscopy
7:02
Analysis
8:24
Conclusion
γH2AX巣に基づいてDNA二本鎖切断の定量は、組織の放射線感受性と放射線修正する化合物の作用の評価に、特に放射線の生物学では、非常に貴重なツールとなっています。ここでは、組織サンプルにおけるγH2AX病巣の定量のための免疫蛍光アッセイの使用方法を示しています。
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