Name: live cell response to mechanical stimulation studied by integrated optical and atomic force microscopy
Uploaded: 2010-10-04T17:00:00
Description: Watch this Scientific Journal Video about Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy at JoVE.com

Plazmid Vinculin-GFP Michael Davidson, Florida Eyalet Üniversitesi, Tallahassee, Florida&#39;daki bir hediye plazmid Ulusal Sağlık Enstitüleri, Ulusal Diş ve Kraniofasial Araştırma Enstitüsü, Bethesda, Maryland ve aktin-MRFP Kenneth Yamada bir hediye oldu. Bu çalışma, NSF Kariyer Ödülü # 0747334 ve AHA-Milli SDG # 0835205N Andreea Trache tarafından desteklenen oldu.

1. Entegre Mikroskop Sistemi Genel Bakış Bu çalışmalar için kullanılan mikroskop sistemi 1 ayrıntılı tarif edilmiştir. Kısaca, TIRF eki (Olympus, Center Valley, PA) ile ters bir Olympus IX-81 mikroskop ile birlikte CSU-22 Yokogawa tarama kafası (Yokogawa Elektrik A.Ş., Japonya). Bioscope SZ atomik kuvvet mikroskobu (Veeco Instruments Inc, Santa Barbara, CA), optik ters mikroskop üstüne monte edilir ve tüm sistemi bir araştırma notu optik tablonun üst (Newport, Ir...

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Sitoiskelet 7-10 ve odak yapışıklıklar 11-16, montajı, dağıtmak ve hücreleri göç veya mekanik güç, 17 cevap olarak teslim dinamik yapılardır . Hücre büyümesi, hayatta kalma ve gen ekspresyonu düzenlenmesinde önemli işlevleri var. Kendi özel dağıtım ve dinamikleri bilmek hücreleri matrisi ve kendi aralarında nasıl iletişim daha iyi anlaşılmasını sağlar. Bu yeni mikroskop sistemi yapışık herhangi bir canlı hücreler molekül...

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		<th>Catalogue Number</th>
		<th>Comment</th>
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<td>AFM tip</td>
<td>&nbsp;</td>
<td>Novascan Technologies</td>
<td>&nbsp;</td>
<td>2 micron Borosilicate bead, 0.01N/m, Biotin Surface</td>
</tr>
<tr>
<td>avidin</td>
<td>&nbsp;</td>
<td>Sigma Aldrich</td>
<td>A9275</td>
<td>1mg/mL in DPBS</td>
</tr>
<tr>
<td>Fibronectin</td>
<td>&nbsp;</td>
<td>Sigma Aldrich</td>
<td>F4759</td>
<td>1 mg/mL</td>
</tr>
<tr>
<td>DPBS</td>
<td>&nbsp;</td>
<td>Invitrogen</td>
<td>21600-051</td>
<td>&nbsp;</td>
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<td>Amaxa transfection kit for Smooth Muscle Cells</td>
<td>&nbsp;</td>
<td>Lonza</td>
<td>VPI-1004</td>
<td>&nbsp;</td>
<tr>
<td>Mattek glass bottom dishes</td>
<td>&nbsp;</td>
<td>MatTek Corporation</td>
<td>P60G-1.5-30-F</td>
<td>Sterile 60mm Falcon dishes with glass bottom coverslip no. 1.5</td>
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live cell response to mechanical stimulation studied by integrated optical and atomic force microscopy

Canlı hücreler mekanik kuvvetlerin anlamda mekanizmasını anlamak ve alt hücresel düzeyde yüksek mekansal ve zamansal çözünürlüğe sahip hücrelerin davranışını araştırmak için tepki ve çevreleriyle uyum nasıl yeni bir teknoloji geliştirildi. Böylece, bir atomik kuvvet mikroskobu (AFM), toplam iç yansıma floresan (TIRF) mikroskopi ve hızlı dönen disk (FSD) konfokal mikroskobu ile entegre edildi. Entegre bir sistem, gerçek zamanlı mekanik uyarılması hücre yanıtları doğrudan optik görüntüleme sağlayan geniş yapışık herhangi bir canlı hücreler moleküler dinamik çalışmaların geniş bir aralığında uygulanabilir. Aktin filamentler ve odak yapışıklıklar önemli düzenlenmesi nedeniyle apikal hücre yüzeyinde hücre gövdesi boyunca hücresel yapıya değişikliklerin neden olduğu yerel mekanik stimülasyonu gösterildi. Bu yenilikçi teknikler, mekanik güç, cevap canlı hücre yeniden yapılanma ve dinamikleri anlamak için yeni bilgiler sağlayacaktır. Ayrıntılı bir protokol ve temsil eden bir veri mekanik stimülasyonu göstermek için canlı hücre yanıtı verildikleri ayarlayın.

Watch this Scientific Journal Video about Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy at JoVE.com

Live Cell Response to Mechanical Stimulation Studied by Integrated Optical and Atomic Force Microscopy

Bu kağıt kültürü canlı hücreleri mekanik uyarılması için entegre bir atom operasyon okuyucu kuvvet optik görüntüleme mikroskop öğretmeyi hedefliyor. Bir adım-adım protokol sunulmuştur. Mekanik stimülasyonu canlı hücre yanıtı gösteren bir temsilci veri seti sunulmaktadır.

Tümleşik Optik ve Atomik Kuvvet Mikroskobu eğitimi Mekanik Uyarım Canlı Hücre Tepki

To understand the mechanism by which living cells sense mechanical forces, and how they respond and adapt to their environment, a new technology able to ...

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