Name: experimental metastasis and ctl adoptive transfer immunotherapy mouse model
Uploaded: 2010-11-26T15:03:00
Description: Watch this Scientific Journal Video about Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model at JoVE.com

由美国国立卫生研究院（CA133085吉隆坡）和美国癌症协会的资助（RSG - 09 - 209 - 01 - TBG的吉隆坡）支持。

1。实验转移小鼠模型<ol><li>前一天的肿瘤细胞的注射剂，种子之一T75容量瓶中，用高达1 × 10 7 CMS4了10 mL的RPMI培养基中含有10％的血清，以获得快速增长的肿瘤细胞中的细胞。过夜孵育在37 ° C。 </li><li>在注射当天，去除培养基，用PBS冲洗细胞一次，然后用0.05％胰酶EDTA收获肿瘤细胞在37 ° C为5分钟。停止与10 mL的RPMI培养基中含有10％血清中反应。细胞转移到锥形管。 </li><li>分拆为3分钟，...

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	<li>Ryan, M. H., Bristol, J. A., McDuffie, E., Abrams, S. I. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Regression+of+extensive+pulmonary+metastases+in+mice+by+adoptive+transfer+of+antigen-specific+CD8(+)+CTL+reactive+against+tumor+cells+expressing+a+naturally+occurring+rejection+epitope.">Regression of extensive pulmonary metastases in mice by adoptive transfer of antigen-specific CD8(+) CTL reactive against tumor cells expressing a naturally occurring rejection epitope.</a> J Immunol. 167 (8), 4286-4292 (2001).</li><li>Caldwell, S. A., Ryan, M. H., McDuffie, E., Abrams, S. 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解决方案： 印度国内的油墨解决方案（17）： <ol><li>倒入150毫升蒸馏水250毫升容量瓶中。 </li><li>加入4滴氢氧化铵的蒸馏水。 </li><li>氨和水的混合物中加入30毫升墨汁的股票（即桑福德魔法防水绘图墨水4465项44011）。 </li><li>顶用蒸馏水到200毫升的量。解决方案是准备注射。 </li></ol>的Fekete的解决方案（17）： 是用来漂白印度虚增墨荷瘤肺的Fekete的解决方案，以区别于正常组织的黑色背景白色的肿瘤结节。 <ol><li> 350毫升95％的乙醇添加1L玻璃瓶。 </li><li>添加150毫升蒸馏水</li><li>添加50毫升甲醛</li><li>添加25毫升冰川酸的酸性物质</li></ol>

experimental metastasis and ctl adoptive transfer immunotherapy mouse model

实验转移小鼠模型是一个简单的，但有关生理转移模型。肿瘤细胞静脉注射（四）为小鼠尾静脉和殖民，从而在肺部，类似肿瘤细胞自发转移的最后步骤：在流通领域，外渗及远端器官的殖民化的生存。从治疗的观点来看，实验转移模型是最简单和理想的模式，自疗法的目标往往是：建立在远端器官的转移瘤的转移终点。在此模型中，肿瘤细胞注射四到小鼠尾静脉，并允许在肺部进行殖民和增长。然后注入肿瘤特异性CTL转移轴承鼠标第四。肺转移的数量和大小，可以控制被注入肿瘤细胞的数量和肿瘤生长的时间。因此，各个阶段的转移，从最小的转移到全身广泛转移，可为蓝本。肺转移，从而允许更容易直观地观察和定量分析与油墨的通货膨胀。

Watch this Scientific Journal Video about Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model at JoVE.com

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

一个实验性肺转移和CTL免疫小鼠模型的肿瘤细胞- T细胞相互作用分析<em&gt;在体内</em&gt;。

实验转移和CTL过继转移免疫小鼠模型

Experimental metastasis mouse model is a simple and yet physiologically relevant metastasis model. The tumor cells are injected intravenously (i.v) into ...

Research

JoVE Journal

Immunology and Infection

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

实验小鼠模型阴道接种和样品采集的协议念珠菌阴道炎

Vulvovaginal candidiasis (VVC), caused by Candida species, is a fungal infection of the lower female genital tract that affects approximately 75% of ...

科研

免疫与感染

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis EAE Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

在实验性自身免疫性脑脊髓炎（EAE）抗性品系小鼠继转移和抗原挑战克服反应迟钝

Experimental autoimmune encephalomyelitis (EAE) is an inflammatory disease of the central nervous system (CNS) and has been used as an animal model for ...

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

加速1型糖尿病感应在小鼠体内的过继转移致糖尿病的CD4 + T细胞

The nonobese diabetic (NOD) mouse spontaneously develops autoimmune diabetes after 12 weeks of age and is the most extensively studied animal model of ...

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

功能研究和过继转移实验小鼠骨髓嗜中性粒细胞的分离，纯化及贴标

Neutrophils are critical effector cells of the innate immune system. They are rapidly recruited at sites of acute inflammation and exert protective or ...

Intralymphatic Immunotherapy and Vaccination in Mice

淋巴免疫治疗和疫苗接种小鼠

Vaccines are typically injected subcutaneously or intramuscularly for stimulation of immune responses. The success of this requires efficient drainage of ...

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

评估小鼠浆细胞树突状细胞在淋巴集结的开发利用造血祖细胞的过继转移

This protocol details a method to analyze the ability of purified hematopoietic progenitors to generate plasmacytoid dendritic cells (pDC) in intestinal ...

Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

CAR性T细胞过继治疗的护理标准胶质母细胞瘤的上下文生成

Adoptive T cell immunotherapy offers a promising strategy for specifically targeting and eliminating malignant gliomas. T cells can be engineered ex vivo ...

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

诱导小鼠肠道炎症由效应CD4的过继转移<sup&gt; +</sup&gt; CD45RB<sup&gt;高</sup&gt; T细胞免疫缺陷小鼠

There are many different animal models available for studying the pathogenesis of human inflammatory bowel diseases (IBD), each with its own advantages ...

Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

立体定向移植人胶质瘤多形性移植小鼠细胞毒性免疫细胞模型的研究

Glioblastoma multiforme (GBM), the most frequent and aggressive primary brain cancer in adults, is generally associated with a poor prognosis, and scarce ...

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

新生儿多药脓毒症的对照小鼠模型

Neonatal sepsis remains a global burden. A preclinical model to screen effective prophylactic or therapeutic interventions is needed. Neonatal mouse ...