Name: experimental metastasis and ctl adoptive transfer immunotherapy mouse model
Uploaded: 2010-11-26T15:03:00
Description: Watch this Scientific Journal Video about Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model at JoVE.com

Ulusal Sağlık Enstitüleri (KL için CA133085) ve Amerikan Kanser Derneği (KL RSG-09-209-01-TBG) hibe ile desteklenir.

1. Deneysel metastaz Fare Modeli <ol><li> Gün önce tümör hücresi enjeksiyonları, 1&#39;e kadar tohum T75 balon x 10 7 CMS4 Met hücreleri RPMI orta hızlı büyüyen tümör hücreleri elde etmek için% 10 serum içeren 10 ml. 37 o gece inkübe ° C </li><li> Enjeksiyon gününde, orta kaldırmak ve hücreler PBS ile bir kez yıkayın, sonra 37 0.05% tripsin-EDTA ile tümör hücrelerinin hasat ° C 5 dakika. 10 ml% 10 serum içeren RPMI orta ile reaksiyon durdurun. Konik bir tüp hücreleri aktarın....

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Çözümler: Hindistan Mürekkep Çözümü (17): <ol><li> 150 ml, 250 ml balona distile su dökün. </li><li> 4 damla amonyum hidroksit, distile su ekleyin. </li><li> 30 ml Hindistan Mürekkep stok (yani Sanford Black Magic su geçirmez Çizim Mürekkep 4465 Öğe 44.011), amonyak ve su karışımı ekleyin. </li><li> 200 ml&#39;lik bir hacim distile su ile kapalı üst. Çözüm enjeksiyon için hazırdır. </li></ol> Fekete Çözümü (17): Fekete çözümü normal dokuların siyah arka plan beyaz tümör nodülü ayırt etmek için ağartıcı Hindistan mürekkep şişirilmiş tümör taşıyan akciğerler için kullanılır. <ol><li> 1L cam şişe, 350ml 95% EtOH ekleyin. </li><li> 150ml distile su ekleyin </li><li> 50ml formaldehit </li><li> 25ml buzul asidik asit ekle </li></ol>

experimental metastasis and ctl adoptive transfer immunotherapy mouse model

Deneysel metastaz fare modeli basit ve fizyolojik ilgili henüz metastaz modeli. Tümör hücrelerinin fare kuyruğu damarlar içine (iv) damar içine enjekte ve tümör hücre spontan metastaz son adımları benzeyen, böylece, akciğerlerde kolonize: distal organlarda kan dolaşımı, ekstravazasyonu ve sömürgeciliğin yaşam. Metastatik tümör distal organ yılında kurulan: tedavilerin hedef genellikle metastaz bitiş noktası bu yana tedavi edici bir açıdan bakıldığında, deneysel metastaz modeli en basit ve ideal bir model. Bu modelde, tümör hücrelerinin fare kuyruğu damarlar içine iv enjekte edilir ve akciğerlerde kolonize ve büyümeye izin. Tümör spesifik CTL sonra iv metastaz taşıyan fare içine enjekte edilir. Akciğer metastazları sayısını ve boyutunu enjekte edilecek tümör hücrelerinin sayısı ve tümör büyümesini zaman kontrol edilebilir. Bu nedenle, çeşitli aşamalarında metastaz, geniş metastaz minimal metastaz modellenmiş olabilir. Akciğer metastazı, böylece daha kolay görsel gözlem ve miktar sağlayarak, mürekkep ile enflasyon analiz edilir.

Watch this Scientific Journal Video about Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model at JoVE.com

Experimental Metastasis and CTL Adoptive Transfer Immunotherapy Mouse Model

Tümör hücrelerinin T hücre etkileşimi analizi için deneysel bir akciğer metastazı ve CTL immünoterapi fare modeli<em&gt; In vivo</em&gt;.

Deneysel metastaz ve CTL Kabul edilmiş Transfer İmmünoterapi Fare Modeli

Experimental metastasis mouse model is a simple and yet physiologically relevant metastasis model. The tumor cells are injected intravenously (i.v) into ...

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Immunology and Infection

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

Protocols for Vaginal Inoculation and Sample Collection in the Experimental Mouse Model of Candida vaginitis

Deneysel Fare Modeli Vajinal Aşılama ve Örnek Toplama Protokolleri Candida Vajinit

Vulvovaginal candidiasis (VVC), caused by Candida species, is a fungal infection of the lower female genital tract that affects approximately 75% of ...

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis (EAE) Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

Overcoming Unresponsiveness in Experimental Autoimmune Encephalomyelitis EAE Resistant Mouse Strains by Adoptive Transfer and Antigenic Challenge

Kabul edilmiş Transfer ve antijenik Challenge Deneysel Otoimmün Encephalomyelitis (EAE) Dayanıklı Fare Suşlarında yanıtsızlık Aşmak

Experimental autoimmune encephalomyelitis (EAE) is an inflammatory disease of the central nervous system (CNS) and has been used as an animal model for ...

Accelerated Type 1 Diabetes Induction in Mice by Adoptive Transfer of Diabetogenic CD4+ T Cells

Diyabetojenik CD4 Kabul edilmiş Transfer ile Farelerde Hızlandırılmış Tip 1 Diyabet İndüksiyon + T Hücreleri

The nonobese diabetic (NOD) mouse spontaneously develops autoimmune diabetes after 12 weeks of age and is the most extensively studied animal model of ...

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

İzolasyon, Arıtma ve İşlevsel Araştırmalar ve Kabul edilmiş Transfer deneyler için Fare Kemik İliği Nötrofiller Etiketlenmesi

Neutrophils are critical effector cells of the innate immune system. They are rapidly recruited at sites of acute inflammation and exert protective or ...

Intralymphatic Immunotherapy and Vaccination in Mice

Fare intralenfatik immünoterapi ve Aşılama

Vaccines are typically injected subcutaneously or intramuscularly for stimulation of immune responses. The success of this requires efficient drainage of ...

Assessing the Development of Murine Plasmacytoid Dendritic Cells in Peyer's Patches Using Adoptive Transfer of Hematopoietic Progenitors

Hematopoetik progenitörlerin Evlatlık Transferi kullanarak Peter Parçacıklannda Murin plazmasitoid dendritik hücreleri Gelişimini değerlendirilmesi

This protocol details a method to analyze the ability of purified hematopoietic progenitors to generate plasmacytoid dendritic cells (pDC) in intestinal ...

Generation of CAR T Cells for Adoptive Therapy in the Context of Glioblastoma Standard of Care

Bakım Glioblastoma Standardı Kapsamında Adoptif Tedavisi için CAR T hücrelerinin üretilmesi

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Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Induction of Murine Intestinal Inflammation by Adoptive Transfer of Effector CD4+CD45RBhigh T Cells into Immunodeficient Mice

Efektör CD4 Evlatlık transferi ile Kemirgen Bağırsak Enflamasyon indüksiyonu<sup&gt; +</sup&gt; CD45RB<sup&gt; Yüksek</sup&gt; Bağışık yetmezliği olan farelere içine T Hücreleri

There are many different animal models available for studying the pathogenesis of human inflammatory bowel diseases (IBD), each with its own advantages ...

Stereotactic Adoptive Transfer of Cytotoxic Immune Cells in Murine Models of Orthotopic Human Glioblastoma Multiforme Xenografts

Orthotopic insan Glioblastoma Multiforme Xenografts fare modelleri sitotoksik bağışıklık hücrelerinin stereotaksik evlat edinen Transfer

Glioblastoma multiforme (GBM), the most frequent and aggressive primary brain cancer in adults, is generally associated with a poor prognosis, and scarce ...

A Controlled Mouse Model for Neonatal Polymicrobial Sepsis

Yenidoğan Polymicrobial Sepsis için kontrollü fare modeli

Neonatal sepsis remains a global burden. A preclinical model to screen effective prophylactic or therapeutic interventions is needed. Neonatal mouse ...