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DOI :
10.3791/2079-v
•
15:55 min
August 4th, 2010
Chapters
0:04
Title
0:58
Introduction
1:29
Microinjection Preparation
3:22
Microinjection
8:00
Fixation and Immunofluorescence
10:02
Plastic Embedding
12:10
Sectioning
Visualizing Nephrotoxic Kidney Damage
14:54
Conclusion
我们描述后fetilization(DPF)的斑马鱼幼虫诱发急性肾损伤(AKI)2天氨基糖甙类抗生素,庆大霉素,显微注射技术。我们还描述了一个整装免疫组织化学方法,塑料包埋,切片和斑马鱼幼虫形象化的AKI的介导的损伤。
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