Name: intravenous microinjections of zebrafish larvae to study acute kidney injury
Uploaded: 2010-08-04T17:32:00
Duration: 15 min 55 s
Description: Watch this Scientific Journal Video about Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury at JoVE.com

<p class="jove_content">この作品は、衛生研究所（NIH）助成金R01DK069403とP30DK079307の米国の国民の協会によって資金を供給された。 </p>

<ol>
	<li>Hentschel, D. M., Park, K. M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Acute+renal+failure+in+zebrafish:+a+novel+system+to+study+a+complex+disease.">Acute renal failure in zebrafish: a novel system to study a complex disease.</a> <em style='font-style: italic'>Am J Physiol Renal Physiol</em>. <b>288</b> (5), F923-F929 (2005).</li><li>Majumdar, A., Lun, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Zebrafish+no+isthmus+reveals+a+role+for+pax2.1+in+tubule+differentiation+and+patterning+events+in+the+pronephric+primordia.">Zebrafish no isthmus reveals a role for pax2.1 in tubule differentiation and patterning events in the pronephric primordia.</a> <em style='font-style: italic'>Development</em>. <b>127</b> (10), 2089-2098 (2000).</li><li>Weinstein, B. M., Stemple, D. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Gridlock,+a+localized+heritable+vascular+patterning+defect+in+the+zebrafish.">Gridlock, a localized heritable vascular patterning defect in the zebrafish.</a> <em style='font-style: italic'>Nat Med</em>. <b>1</b> (11), 1143-1147 (1995).</li><li>Westerfield, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>The Zebrafish Book</em>. , (1993).</li></ol>

利害の衝突は宣言されません。

<p class="jove_content">このビデオの記事では、ゼブラフィッシュ幼生におけるゲンタマイシンの媒介近位尿細管の損傷を作成することで、AKIのモデルのための静脈内マイクロインジェクション法を示した。心膜および/または総浮腫の形成にこの損傷は、その結果、水のバランスを調節することができないことを反映。我々はまた、細胞極性の喪失や損傷した腎臓の近位尿細管の混乱を示す、差別化された腎臓の尿細管を（マジュムダール<em>ら、2000）</em>マーク抗体による免疫組織化学実験を説明した。静脈内microinjectionsは、ゼブラフィッシュ幼生の血流中に水溶性物質を提供するための効率的な方法を表しています。この手法は、様々な物質を導入するための優れたツールであり、蛍光マーカーのユニフォームの注射の助けを借りて、一貫性のある結果を可能にする、何度も繰り返すことができます。 </p>

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intravenous microinjections of zebrafish larvae to study acute kidney injury

<p class="jove_content">このビデオの記事では、nephrotoxicantとしてゲンタマイシンを使用してAKIのゼブラフィッシュモデルを説明します。テクニックは、2 DPFのゼブラフィッシュでの静脈内microinjectionsで構成されています。この手法は、時間あたり15〜20魚の注入を可能に、ゼブラフィッシュ幼生の血流中に可溶性物質を提供する効率的で迅速な方法を表します。 AKIの研究に加えて、このマイクロインジェクション技術はまた、血管造影などの実験研究の他のタイプに使用することができます。我々は減少し、腎機能の視覚的な措置に必要な機器からの技術の詳細なプロトコルを提供しています。さらに、我々はまた、腎臓の尿細管マーカーで幼生ゼブラフィッシュの埋め込みプラスチックと切片を固定、全体のマウント免疫組織化学のプロセスを示しています。浮腫、近位尿細管上皮細胞における細胞極性の喪失、および尿細管の形態学的混乱：私たちは、ゲンタマイシンを注射したゼブラフィッシュの幼虫は、一貫性のあるAKIとの形態的特徴を示して実証する。</p>

Watch this Scientific Journal Video about Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury at JoVE.com

Intravenous Microinjections of Zebrafish Larvae to Study Acute Kidney Injury

<p class="jove_content">我々は2日後fetilization（DPF）急性腎障害（AKI）を誘導するためにゼブラフィッシュの幼虫にアミノグリコシド、ゲンタマイシンを、マイクロインジェクションのテクニックを説明します。我々はまた、AKIの媒介の損傷を可視化するためにゼブラフィッシュ幼生の埋め込みプラスチックと切片全体のマウント免疫組織化学のための方法を、説明します。</p>

急性腎障害を研究するためにゼブラフィッシュ幼生の静脈内Microinjections

In this video article we describe a zebrafish model of AKI using gentamicin as the nephrotoxicant.  The technique consists of intravenous microinjections ...

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