Name: tri-layered electrospinning to mimic native arterial architecture using polycaprolactone, elastin, and collagen: a preliminary study
Uploaded: 2011-01-04T16:00:00
Description: Watch this Scientific Journal Video about Tri-layered Electrospinning to Mimic Native Arterial Architecture using Polycaprolactone, Elastin, and Collagen: A Preliminary Study at JoVE.com

<p class="jove_content">我们要感谢美国心脏协会中大西洋联盟（0555407U，GLB）提供资金。 </p>

<ol><li>静电之前，胶原蛋白，从6个月的牛真皮，必须提取使用醋酸化进程。这提取的胶原蛋白，然后通过随后的一系列解散，降水，并dialyses纯化。一旦胶原蛋白冻干粉，静电纺丝的过程就可以开始。 </li><li>三种材料集结：聚己内酯（PCL），弹性（ELAS）和胶原蛋白（COL）。这些材料将被分别溶解六氟-2 - 丙醇（HFP，特克斯和凯科斯群岛美）的浓度在100毫克/毫升，200毫克/毫升和70毫克/毫升，分别在1,1,1,3,3,3。 </li><li...

<ol>
	<li>McClure, M. J., Sell, S. A., Barnes, C. P., Bowen, W. C., Bowlin, G. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cross-linking+Electrospun+Polydioxanone-Soluble+Elastin+Blends:+Material+Characterization.">Cross-linking Electrospun Polydioxanone-Soluble Elastin Blends: Material Characterization.</a> <em style='font-style: italic'>Journal of Engineered Fibers and Fabrics</em>. <b>3</b>, 1-10 (2008).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> <em style='font-style: italic'>Cardiovascular implants - Vascular graft protheses</em>. <b>VP20</b>, (1994).</li><li>McClure, M. J., Sell, S. A., Simpson, D. G., Walpoth, B. H., Bowlin, G. L. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+three-layered+electrospun+matrix+to+mimic+native+arterial+architecture+using+polycaprolactone,+elastin,+and+collagen:+A+preliminary+study.">A three-layered electrospun matrix to mimic native arterial architecture using polycaprolactone, elastin, and collagen: A preliminary study.</a> <em style='font-style: italic'>Acta Biomater</em>. , (2010).</li></ol>

<p class="jove_content">这项研究的最关键部分是静电纺丝的过程。当使用3-1输入输出喷嘴，拱架和可能发生电荷损失。如果这确实发生，纺丝与聚合物，是相关联的电压将下降，造成焊接，“湿”的纤维和创造之间的三层分层。因此，一致的电势，都必须获得一个理想的多层管。 </p><p class="jove_content">遵守不匹配是在小直径的移植物闭塞的主要原因之一。发展多层次的血管移植，提供了对的东西，可以模仿自然的生物?...

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		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>Polycaprolactone</td>
<td>&nbsp;</td>
<td>Sigma</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Elastin</td>
<td>&nbsp;</td>
<td>Elastin Products Company</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Collagen</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Acetic Acid</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Sodium Chloride</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>TRIS</td>
<td>&nbsp;</td>
<td>Fisher</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>6 L Avanti J-HC Centrifuge</td>
<td>&nbsp;</td>
<td>Beckman Coulter</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>1,1,1,3,3,3 hexafluoro-2-propanol</td>
<td>&nbsp;</td>
<td>TCI America</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>3 ml Becton Dickinson Syringe</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>CZE1000R Rack Mount Power Supply</td>
<td>&nbsp;</td>
<td>Spellman</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>High-Pressure Syringe Pump</td>
<td>&nbsp;</td>
<td>Cole Parmer</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide</td>
<td>&nbsp;</td>
<td>Pierce</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>70 % ethanol</td>
<td>&nbsp;</td>
<td>Sigma Aldrich</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>ImageTool 3.0 software</td>
<td>&nbsp;</td>
<td>Shareware provided by UTHSCSA</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Scanning Electron Microscope</td>
<td>&nbsp;</td>
<td>Zeiss EV050</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>MTS Bionix 200 testing system</td>
<td>&nbsp;</td>
<td>MTS Systems Corp.</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>TestWorks version 4</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<td>&nbsp;</td>
<tr>
<td>Intelligent Tissue Engineering via Mechanical Stimulation (ITEMS) Bioreactor</td>
<td>&nbsp;</td>
<td>Tissue Growth Technologies</td>
<td>&nbsp;</td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>
	

tri-layered electrospinning to mimic native arterial architecture using polycaprolactone, elastin, and collagen: a preliminary study

<p class="jove_content">整个本地动脉，胶原蛋白和弹性蛋白发挥了重要作用，提供了一个机械的骨干，防止血管破裂，促进搏动变形下的恢复。这项研究的目的是模仿编造一个多层次的静电导管组成的聚（己内酯）（PCL）与弹性蛋白和胶原蛋白55-35 45-45-10混纺，除了原生动脉的结构 - 10，和65-25-10的PCL - ELAS COL的演示表明本地动脉组织的力学性能，而其余的有利于组织的再生。整个移植和个人层分析用单轴拉伸试验，动态顺应性，缝合保留，爆破强度。合规结果显示，中间/内侧层的变化改变了整体移植的行为与整个移植遵守值范围从0.8 - 2.8％/ 100毫米汞柱，而单轴结果表明平均模量范围2.0 - 11.8兆帕。模量和遵守数据显示本土动脉范围内的值。数学建模是实现显示层刚度的变化如何影响整体圆周壁应力，并作为设计辅助材料，以达到最佳的机械组合。总的来说，结果表明，移植可以被用来模仿一个三层次结构，通过改变层的属性。 </p>

Watch this Scientific Journal Video about Tri-layered Electrospinning to Mimic Native Arterial Architecture using Polycaprolactone, Elastin, and Collagen: A Preliminary Study at JoVE.com

Tri-layered Electrospinning to Mimic Native Arterial Architecture using Polycaprolactone, Elastin, and Collagen: A Preliminary Study

<p class="jove_content">本研究的目的是模仿原生三个动脉壁的分层结构。静电而要做到这一点，是雇用与使用以3-1（输入输出）喷嘴和聚己内酯，弹性和胶原混合。</p>

三层次的静电，以模仿母语动脉结构采用聚己内酯，弹性和胶原的初步研究

Throughout native artery, collagen and elastin play an important role, providing a mechanical backbone, preventing vessel rupture, and promoting recovery ...

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An Improved Method for the Preparation of Type I Collagen From Skin

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Planar Gradient Diffusion System to Investigate Chemotaxis in a 3D Collagen Matrix

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Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered <em>In Vitro</em> Model of the Airway Wall

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用无标签荧光显微镜评价活体、人体阻力动脉中胶原蛋白和弹性蛋白的压力依赖性 Microarchitectures

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