Name: in vivo micro-circulation measurement in skeletal muscle by intra-vital microscopy
Uploaded: 2007-05-28T00:00:00
Description: Watch this Scientific Journal Video about In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy at JoVE.com

<p>我们感谢他的意见JW李奇曼在体内的肌肉观察。 </p>

<p class="jove_step"> RBC膜染色</p><ol><li>与肝素相同的小鼠品系小鼠红细胞纠正。 </li><li>小鼠红细胞染色PKH26染料（五百六十七分之五百五十一nm的红色荧光细胞连接器套件，美国Sigma公司）。 </li><li>在PBS洗涤红细胞和2μm的溶液在室温下5分钟的PKH26染料孵育。反应是停止通过增加血浆（热灭活1小时在65℃事前）和孵育1分钟。 </li><li>染色用PBS洗涤红细胞的5倍。 </li></ol><p class="jove_step">鼠标的制备</p><ol><li>在这项研究中分?...

<ol>
	<li>Lichtman, J. W., Magrassi, L., Purves, D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Visualization+of+neuromuscular+junctions+over+periods+of+several+months+in+living+mice.">Visualization of neuromuscular junctions over periods of several months in living mice.</a> <em style='font-style: italic'>J Neurosci</em>. <b>7</b>, 1215-1222 (1987).</li></ol>

<p>重要技术资料的要点如下：（1）观察维护动物的生理状况（通风，pH值perfusative解决方案，体温），（2）染色红细胞的注射量，（3）条件（最佳镜头选择，荧光强度）。未来潜在的应用如下：（一）结合pharmacophysiological和/或分子生物学干预措施，（二）长期观察动脉硬化/ arteriolo硬化和新生血管。 </p>

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<td>PKH26 dye </td>
<td>&nbsp;</td>
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<td>551/567 nm; Red fluorescent cell linker kit</td>
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<td>C57BL/10 mice </td>
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<td>Three to six month old males</td>
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<td>pentobarbital </td>
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in vivo micro-circulation measurement in skeletal muscle by intra-vital microscopy

<p class="jove_content">背景：监管因素和体内微循环的详细生理仍然没有完全澄清后成像发明了许多不同的方式。虽然许多宏观参数的血流量反映流速，血流速度和血红细胞（RBC）的变化通量不持有在显微镜观察的线性关系。有报告的差异之间的红细胞流速和红细胞通量，红细胞磁通和血浆流量，并在显微镜下观察，一个更详细的研究在微循环调节的要求的科学依据，使用的外围组织的时空异质性的流量调节活体显微镜。 </p><p class="jove_content">方法：我们在显微镜下观察体内鼠标胸锁乳突肌肌肉修改杰夫李奇曼的方法。小鼠麻醉后，通风，并注射PKH26L -荧光标记的红细胞显微镜观察。 </p><p class="jove_content">结果与结论：荧光标记的红细胞是由宽视场显微镜检测和区分。电刺激诱导的红细胞通量增加肌肉的收缩诱发。包括红细胞流速和毛细血管密度其它参数的量化是可行的。小鼠的耐受性以及手术，注射染色红细胞，显微镜下观察，和电刺激。没有肌肉或血管损伤，这表明我们的方法是侵入性和长期观察，比较适合。 </p>

Watch this Scientific Journal Video about In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy at JoVE.com

In vivo Micro-circulation Measurement in Skeletal Muscle by Intra-vital Microscopy

<p class="jove_content">提出一种新的微循环观察通用的方法。它被认为是适合长期观察，并结合pharmacophysiological或分子生物学干预。</p>

在体内微循环在骨骼肌内重要的显微镜测量

BACKGROUND: Regulatory factors and detailed physiology of in vivo microcirculation have remained not fully clarified after many different modalities of ...

Research

JoVE Journal

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科研

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Maintaining homeostatic Ca2+ signaling is a fundamental physiological process in living cells. Ca2+ sparks are the elementary units of Ca2+ signaling in ...

Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays

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<em>In vivo</em> Clonal Tracking of Hematopoietic Stem and Progenitor Cells Marked by Five Fluorescent Proteins using Confocal and Multiphoton Microscopy

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We developed and validated a fluorescent marking methodology for clonal tracking of hematopoietic stem and progenitor cells (HSPCs) with high spatial and ...

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Live-cell imaging is a microscopy technique used to examine cell and protein dynamics in living cells. This imaging method is not toxic, generally does ...

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