Name: murine echocardiography and ultrasound imaging
Uploaded: 2010-08-08T19:00:00
Description: Watch this Scientific Journal Video about Murine Echocardiography and Ultrasound Imaging at JoVE.com

资助来源：HL084087，HL089885，HL091475，S10RR027946，T32 GM07356

1。成像的制备<ol><li>异氟醚麻醉鼠标的动物仰卧位处理平台的开始。放置一个以上的动物提供0.5-1％异氟醚维持麻醉的鼻子和嘴巴的鼻锥。 </li><li>鼠标的爪子安全进行凝胶电极片。确保适当的心电图，身体的温度在37℃，呼吸频率，在成像检查生理评估。 </li><li>脱毛霜鼠标的胸部和上腹部。 </li><li> 2分钟后，用湿纱布取出的奶油。 </li></ol> 2。左胸骨旁长轴<ol><li>一旦鼠标已?...

<ol>
	<li>Patten, R. D., Hall-Porter, M. R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Small+animal+models+of+heart+failure:+development+of+novel+therapies,+past+and+present.">Small animal models of heart failure: development of novel therapies, past and present.</a> Circ Heart Fail. 2, 138-144 (2009).</li><li>Li, Y. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Quantification+and+MRI+Validation+of+Regional+Contractile+Dysfunction+in+Mice+Post+Myocardial+Infarction+Using+High+Resolution+Ultrasound.">Quantification and MRI Validation of Regional Contractile Dysfunction in Mice Post Myocardial Infarction Using High Resolution Ultrasound.</a> Ultrasound in Medicine &#38; Biology. 33, 894-904 (2007).</li><li>Gardin, J. M., Siri, F. M., Kitsis, R. N., Edwards, J. G., Leinwand, L. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Echocardiographic+Assessment+of+Left+Ventricular+Mass+and+Systolic+Function+in+Mice.">Echocardiographic Assessment of Left Ventricular Mass and Systolic Function in Mice.</a> Circ Res. 76, 907-914 (1995).</li><li>JG, S. t. e. v. e. n. s. o. n., Weiler, T. B. M., EA, H. o. w. a. r. d., Eyer, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Digital+multigate+Doppler+with+color+echo+and+Doppler+display+-+Diagnosis+of+atrial+and+ventricular+septal+defects.">Digital multigate Doppler with color echo and Doppler display - Diagnosis of atrial and ventricular septal defects.</a> Circulation. 60, (1979).</li><li>Patten, R. D., Aronovitz, M. J., Bridgman, P., Pandian, N. G. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Use+of+pulse+wave+and+color+flow+Doppler+echocardiography+in+mouse+models+of+human+disease.">Use of pulse wave and color flow Doppler echocardiography in mouse models of human disease.</a> J Am Soc Echocardiogr. 15, 708-714 (2002).</li><li>Hoit, B. D. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Echocardiographic+characterization+of+the+cardiovascular+phenotype+in+rodent+models.">Echocardiographic characterization of the cardiovascular phenotype in rodent models.</a> Toxicol Pathol. 34, 105-110 (2006).</li></ol>

串行高频超声心动图已经成为一个非侵入性的，高分辨率的心脏形态和功能的评估，特别是在心脏病的小鼠模型，功能强大的工具。保持生理体温以及之前获得的图像通过调整麻醉水平的小鼠之间的可比性心率，这种方法的成功是至关重要的。应使用相同的生物标志物（如胸骨旁乳头肌的长轴成像）获得的所有图像。最后，图像分析，应以一致的方式（例如，一个单一的，蒙蔽的调查），以确保?...

A correction was made to <a href="http://www.jove.com/index/details.stp?id=2100">Murine echocardiography and ultrasound imaging</a>. There was an error in the author, Burns Blaxalls', name and the article title. The authors name has been corrected to:
Burns C. Blaxall
instead of:
Burns Blaxall
The article title was changed to:
Murine Echocardiography and Ultrasound Imaging
instead of:
Murine echocardiography and ultrasound imaging

A correction to an author's name and the article title has been made for the article Murine echocardiography and ultrasound imaging.

Erratum: Murine echocardiography and ultrasound imaging

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murine echocardiography and ultrasound imaging

心脏的病理生理的啮齿类动物模型代表一个有价值的研究工具，调查机制，疾病以及测试新疗法1超声心动图提供了强大的，非侵入性工具，连续评估一个活生生的动物的心脏形态学和功能。，但是，使用这种技术对小鼠对由于这些动物的体积小，心率加快，独特的挑战。直到最近，很少超声系统能够执行质量超声心动图对小鼠，和那些普遍缺乏的图像分辨率和帧速率要获得真正的定量测量。新发布的系统，如VisualSonics Vevo2100为研究者提供新的工具，以谨慎和非侵入性的调查小鼠的心脏功能。该系统生成高分辨率的图像，并提供与人类患者所使用的类似的分析能力。虽然彩色多普勒已超过30年在人类的，这个宝贵的技术最近才得以在啮齿类动物超声。4,5彩色多普勒超声心动图，具有广阔的应用前景，包括能够快速评估船只在通过阀门的流量方向性，并迅速查明瓣关闭不全。应变分析是一个重要的的进步，是利用定量测量区域的心肌功能 。6这项技术有可能检测病理变化，或病理的决议，较常规技术。再加此外，整个器官的三维图像重建容积评估是可能的，包括心脏和血管结构的可视化和评估。鼠兼容相衬成像，还可以让体积测量和组织灌注的评估。

Watch this Scientific Journal Video about Murine Echocardiography and Ultrasound Imaging at JoVE.com

Murine Echocardiography and Ultrasound Imaging

本视频演示了利用轨安装的高频超声探头上执行麻醉小鼠的超声心动图。除了更新，更强大的工具，如彩色多普勒，应变分析，以及一般和有针对性的对比成像的方法描述这两个传统的二维和M型测量心脏功能。

小鼠超声心动图和超声成像

Rodent models of cardiac pathophysiology represent a valuable research tool to investigate mechanism of disease as well as test new therapeutics.1  ...

Research

JoVE Journal

Medicine

Transthoracic Echocardiography in Mice

经胸超声心动图在小鼠

In recent years, murine models have become the primary avenue for studying the molecular mechanisms of cardiac dysfunction resulting from changes in gene ...

科研

医学

Murine Fetal Echocardiography

小鼠胎儿超声心动图

Transgenic mice displaying abnormalities in  cardiac development and function represent a powerful tool for the understanding  the molecular mechanisms ...

Fetal Echocardiography and Pulsed-wave Doppler Ultrasound in a Rabbit Model of Intrauterine Growth Restriction

在家兔模型宫内生长受限胎儿超声心动图和多普勒超声脉冲波

Fetal intrauterine growth restriction (IUGR) results in abnormal cardiac function that is apparent antenatally due to advances in fetoplacental Doppler ...

A Novel Application of Musculoskeletal Ultrasound Imaging

肌肉骨骼超声成像的一种新应用

Ultrasound is an attractive modality for imaging muscle and tendon  motion during dynamic tasks and can provide a complementary methodological  approach ...

Diagnostic Ultrasound Imaging of Mouse Diaphragm Function

小鼠膈肌功能诊断超声成像

Function analysis of rodent respiratory skeletal muscles, particularly the diaphragm, is commonly performed by isolating muscle strips using invasive ...

High-frequency Ultrasound Imaging of Mouse Cervical Lymph Nodes

鼠标颈淋巴结高频超声成像

High-frequency ultrasound (HFUS) is widely employed as a non-invasive method for imaging internal anatomic structures in experimental small animal ...

Murine Echocardiography of Left Atrium, Aorta, and Pulmonary Artery

左心房，主动脉和肺动脉的超声心动图小鼠

The present methodology teaches the investigator how to measure and use the LAV as a surrogate of chronic elevations in Left Ventricular diastolic ...

Quantitative Visualization of Leukocyte Infiltrate in a Murine Model of Fulminant Myocarditis by Light Sheet Microscopy

通过轻型显微镜对小鼠急性心肌炎模型中白细胞浸润的定量可视化

Light-sheet fluorescence microscopy (LSFM), in combination with chemical clearing protocols, has become the gold standard for analyzing fluorescently ...

Providing Visual Biofeedback Using Brightness Mode Ultrasound During a Golf Swing

在高尔夫挥杆期间使用亮度模式超声波提供视觉生物反馈

Using ultrasound biofeedback in conjunction with verbal cueing can increase muscle thickness more than verbal cueing alone and may augment traditional ...

Muscle Function Obtained with Motion Mode Ultrasound and Surface Electromyography during Core Endurance Exercise

核心耐力运动期间通过运动模式超声和表面肌电图获得的肌肉功能

Motion mode (M-mode) ultrasound allows researchers and clinicians to measure the change of muscle thickness across time. Muscle thickness can be measured ...