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DOI :
10.3791/2101-v
•
11:19 min
July 7th, 2010
Chapters
0:00
Title
0:53
Introduction
1:33
Cell Cross-linking
2:39
Cell Sonication
4:03
Chromatin Immunoprecipitation (ChIP)
7:06
Amplification of Genomic DNA
8:27
Gel Purification of Amplified Products
9:46
Representative Results for Genome-wide Analysis Using ChIP
10:53
Conclusion
在这里,我们提出的全基因组的不同在一个组蛋白结合结构域的蛋白异构体的位置分析染色质免疫沉淀(ChIP)的过程。我们把它应用到芯片的SEQ分析,以确定KDM5A/JARID1A/RBP2组蛋白去甲基化酶的目标。
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