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DOI :
10.3791/2134-v
December 3rd, 2010
Chapters
0:05
Title
0:51
Introduction
1:08
Protein Overexpression
2:48
Harvesting and Extracting the Cells
4:58
Preparing the Membrane Fraction and Solubilizing Transmembrane Proteins
6:46
Hexahistidine Tag Affinity Purification by IMAC
8:23
Gel Filtration
9:44
Representative Results from MexB Purification
10:23
Conclusion
在这个协议中,我们展示的表达,增溶,并纯化了重组表达的膜蛋白,MexB,作为一种可溶性蛋白的洗涤剂复杂。 MexB是机会主义的细菌病原体绿脓杆菌多药耐药膜转运。
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