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DOI :
10.3791/2134-v
December 3rd, 2010
Chapters
0:05
Title
0:51
Introduction
1:08
Protein Overexpression
2:48
Harvesting and Extracting the Cells
4:58
Preparing the Membrane Fraction and Solubilizing Transmembrane Proteins
6:46
Hexahistidine Tag Affinity Purification by IMAC
8:23
Gel Filtration
9:44
Representative Results from MexB Purification
10:23
Conclusion
이 프로토콜에서 우리는 가용성 단백질 세제 복합으로 표현, solubilization, 그리고 recombinantly 표현 막 단백질, MexB의 정화를 보여줍니다. MexB는 기회 세균성 병원체 녹농균에서 multidrug 저항 막 수송이다.
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