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DOI :
10.3791/2137-v
•
8:20 min
August 25th, 2010
Chapters
0:00
Title
0:58
Introduction
1:19
RNA Interference in 384-well Plates
3:09
Infecting with Vaccinia Virus
4:11
Staining Plates
6:02
Representative Images and Interpretation of Infection Rates
7:35
Conclusion
在病毒感染的新型主机所涉及的因素可以识别通过细胞为基础的的全基因组功能RNAi筛选的损失。一果蝇细胞培养模型的易用性和效率的RNAi由于这种方法特别适合。在这里,我们展示了这种技术使用牛痘病毒为例。
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