Name: contrast ultrasound targeted treatment of gliomas in mice via drug-bearing nanoparticle delivery and microvascular ablation
Uploaded: 2010-12-15T17:30:00
Description: Watch this Scientific Journal Video about Contrast Ultrasound Targeted Treatment of Gliomas in Mice via Drug-Bearing Nanoparticle Delivery and Microvascular Ablation at JoVE.com

NIH R01 HL74082, 하트웰 재단과 집중 초음파 수술 재단 지원.

1. Microbubble 제작 <ol><li> 알부민 microbubbles을 (MBS) 준비하려면, 수성 단계 위의 가스의 담요 (octafluoropropane)와 플라스크에 정상 생리에 혈청 알부민의 1 % 용액을 넣으십시오. 간단히 확장 ½ &quot;티타늄 프로브가 장착된 초음파 분쇄기로 솔루션 (30 초) sonicate.이 제제는 0.5-1.2 X 10 9 MBS / ML의 농도 범위에서 제공하는 Optison (GE Heathcare)과 유사합니다. Multisizer 쿨터 카운터 메가바이트 직경을 의미...

<ol>
	<li>Chappell, J., Song, J., Burke, C., Klibanov, A., Price, R. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+delivery+of+nanoparticles+bearing+fibroblast+growth+factor-2+by+ultrasonic+microbubble+destruction+for+therapeutic+arteriogenesis.">Targeted delivery of nanoparticles bearing fibroblast growth factor-2 by ultrasonic microbubble destruction for therapeutic arteriogenesis.</a> Small. 10, 1769-1777 (2008).</li><li>Chomas, J. E., Pollard, R., Wisner, E., Ferrara, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Subharmonic+Phase-Inversion+for+Tumor+Perfusion+Estimation.">Subharmonic Phase-Inversion for Tumor Perfusion Estimation.</a> IEEE. 2, 1713-1716 (2001).</li><li>Davda, J., Labhasetwar, V. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Characterization+of+nanoparticle+uptake+by+endothelial+cells.">Characterization of nanoparticle uptake by endothelial cells.</a> Int J Pharm. 233, 51-51 (2002).</li><li>Hynynen, K., McDannolod, N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Noninvasive+MR+imaging-guided+focal+opening+of+the+blood-brain+barrier+in+rabbits.">Noninvasive MR imaging-guided focal opening of the blood-brain barrier in rabbits.</a> Radiology. 220, 640-646 (2001).</li><li>McDannold, N., Vykhodtseva, N., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Microbubble+contrast+agent+with+focused+ultrasound+to+create+brain+lesions+at+low+power+levesl:+MR+imaging+and+histological+study+in+rabbits.">Microbubble contrast agent with focused ultrasound to create brain lesions at low power levesl: MR imaging and histological study in rabbits.</a> Radiology. 241, 95-106 .</li><li>McDannold, N., Vykhodtseva, N., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Targeted+disruption+of+the+blood-brain+barrier+with+focused+ultrasound:+Association+with+cavitation+activity.">Targeted disruption of the blood-brain barrier with focused ultrasound: Association with cavitation activity.</a> Phys Med Biol. 51, 793-807 .</li><li>McDannold, N., Clement, G. T., Black, P., Jolesz, F., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Transcranial+magnetic+resonance+imaging-+guided+focused+ultrasound+surgery+of+brain+tumors:+initial+findings+in+3+patients.">Transcranial magnetic resonance imaging- guided focused ultrasound surgery of brain tumors: initial findings in 3 patients.</a> Neurosurgery. 66, 323-332 (2009).</li><li>Meairs, S., Alonso, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Ultrasound+microbubblesand+the+blood+brain+barrier.">Ultrasound microbubblesand the blood brain barrier.</a> Progr Biophys Mol Biol. 93, 354-362 (2007).</li><li>. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=.">.</a> Optison, products insert. , (2009).</li><li>Sadlowskie, A., Chromas, J., Pollard, R., Bloch, S., Griffey, S., Wisner, W., Ferrara, K. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Mean+Flow+Rate+and+Intergrated+Perfusion+Estimates+Obtained+with+Contrast-Assisted+Ultrasound.">Mean Flow Rate and Intergrated Perfusion Estimates Obtained with Contrast-Assisted Ultrasound.</a> IEEE Ultrasonics Symposium. , 1977-1980 (2002).</li><li>Song, J., Chappell, J. C., Qi, M., VanGieson, E. J., Kaul, S., Price, R. J. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Influence+of+injection+site,+microvascular+pressure+and+ultrasound+variables+on+microbubble-mediated+delivery+of+microspheres+to+muscle.">Influence of injection site, microvascular pressure and ultrasound variables on microbubble-mediated delivery of microspheres to muscle.</a> J Am Coll. Cardiol. 39, 726-731 (2002).</li><li>Sheikov, N., McDannold, N., Vykhodtseva, N., Jolesz, F., Hynynen, K. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Cellular+mechanisms+of+blood-brain+barrier+opeing+induced+by+ultrasound+in+the+presences+of+microbubbles.">Cellular mechanisms of blood-brain barrier opeing induced by ultrasound in the presences of microbubbles.</a> Ultrasound Med. Biol. 30, 979-989 (2004).</li><li>Tanter, J., Fink, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Focusing+and+steering+through+absorbing+and+aberrating+layers:+Application+to+ultrasonic+propagation+through+the+skull.">Focusing and steering through absorbing and aberrating layers: Application to ultrasonic propagation through the skull.</a> J Acoust Soc Am. 103, 2403-2410 (1998).</li><li>Yeh, C. K., Kruse, D. E., Lim, M. C., Redline, D. E., Ferrara, K. W. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+New+High+Frequency+Destruction/Reperfusion+System.">A New High Frequency Destruction/Reperfusion System.</a> IEEE. 1, 433-436 (2003).</li></ol>

중요 단계 마우스 꼬리 정맥의 Cannulation : 마우스 꼬리 정맥에 정맥 주사는 도전적인 절차하실 수 있습니다. 그러나, 꼬리 정맥 카테터는 크게 성공적인 주사의 가능성을 향상시킬 수 있습니다. 그것은 허브에서 휴식까지 카테터를하기 위해서 반복적으로 앞뒤로 25 게이지 바늘을 구부. PE 20 튜브의 끝 부분에 뭉툭한 끝부분을 삽입하고 실리콘 접착제로 연결...

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		<div>
		<table border="1">
		<thead>
		<tr>
		<th>Material Name</th>
		<th>Type</th>
		<th>Company</th>
		<th>Catalogue Number</th>
		<th>Comment</th>
		</tr>
		</thead>
		<tbody>
		
<tr>
<td>ApoptTag kit</td>
<td>&nbsp;</td>
<td>Intergen Co.</td>
<td>S7110</td>
<td>&nbsp;</td>
<tr>
<td>un-capped 85:15 poly(lactic-co-glycolic acid) (PLAGA)</td>
<td>&nbsp;</td>
<td>Lakeshore Biomaterials</td>
<td>Custom</td>
<td>&nbsp;</td>
<tr>
<td>Vivo Tag 680</td>
<td>&nbsp;</td>
<td>VisEn Medical</td>
<td>10120</td>
<td>Used to Tag BSA</td>
</tr>
<tr>
<td>Poly(vinyl alcohol)</td>
<td>&nbsp;</td>
<td>Sigma-Aldrich</td>
<td>363136</td>
<td>&nbsp;</td>
<tr>
<td>MicroTip Sonicator</td>
<td>&nbsp;</td>
<td>Misonix</td>
<td>S-4000</td>
<td>&nbsp;</td>
<tr>
<td>Sequoia</td>
<td>&nbsp;</td>
<td>Simons Medical</td>
<td>P.O.A</td>
<td>Equipped with CPS </td>
</tr>
<tr>
<td>FreeZone 2.5</td>
<td>&nbsp;</td>
<td>Labconco</td>
<td>7670020</td>
<td>Equipped with Nitrogen Trap</td>
</tr>
<tr>
<td>Methylene chloride (CH2Cl2)</td>
<td>&nbsp;</td>
<td>Fisher Scientific</td>
<td>D37-500</td>
<td>&nbsp;</td>
<tr>
<td>FMT 250</td>
<td>&nbsp;</td>
<td>VisEn Medical</td>
<td>P.O.A</td>
<td>&nbsp;</td>
<tr>
<td>F-12K Nutrient Mixture</td>
<td>&nbsp;</td>
<td>Gibco</td>
<td>21127-022</td>
<td>&nbsp;</td>
<tr>
<td>polyethyleneglycol-40 stearate </td>
<td>&nbsp;</td>
<td>Sigma Chemical </td>
<td>9004-99-3</td>
<td>&nbsp;</td>
<tr>
<td>distearoyl phosphatidylcholine</td>
<td>&nbsp;</td>
<td>Avanti Polar Lipids</td>
<td>770365</td>
<td>&nbsp;</td>
<tr>
<td>Multisizer Coulter Counter</td>
<td>&nbsp;</td>
<td>Beckman Coulter </td>
<td>P.O.A</td>
<td>&nbsp;</td>
<tr>
<td>Waveform Generator </td>
<td>&nbsp;</td>
<td>Tektronix, Inc.</td>
<td>AFG-310</td>
<td>&nbsp;</td>
<tr>
<td>water-based ultrasound gel </td>
<td>&nbsp;</td>
<td>Parker Laboratories</td>
<td>Aquasonic 100</td>
<td>&nbsp;</td>
<tr>
<td>Infusion pump </td>
<td>&nbsp;</td>
<td>Harvard Apparatus</td>
<td>Harvard Apparatus PHD 2000</td>
<td>&nbsp;</td>
<tr>
<td>1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) </td>
<td>&nbsp;</td>
<td>Pierce Biotechnology</td>
<td>25952-53-8</td>
<td>&nbsp;</td>
<tr>
<td>N-hydroxysulfosuccinimide (Sulfo-NHS)</td>
<td>&nbsp;</td>
<td>Pierce Biotechnology</td>
<td>106627-54-7</td>
<td>&nbsp;</td>
<tr>
<td>Succinic anhydride</td>
<td>&nbsp;</td>
<td>Sigma Aldrich</td>
<td>603902</td>
<td>&nbsp;</td>
<tr>
<td>Power Amplifier </td>
<td>&nbsp;</td>
<td>Electronic Navigation Industries</td>
<td>ENI 3100LA</td>
<td>&nbsp;</td>
<tr>
<td>Needle Thermocouple Probe </td>
<td>&nbsp;</td>
<td>Omega</td>
<td>HYP1-30-1/2-T-G-60-SMPW-M</td>
<td>&nbsp;</td>
<tr>
<td>BioGel (P100, medium)</td>
<td>&nbsp;</td>
<td>Bio-Rad</td>
<td>150-4170</td>
<td>&nbsp;</td>
<tr>
<td>.75&#x2019;&#x2019; diameter 1 MHz unfocused transducer</td>
<td>&nbsp;</td>
<td>Panametrics</td>
<td>A314S </td>
<td>&nbsp;</td>		</tbody>
		</table>
		</div>

contrast ultrasound targeted treatment of gliomas in mice via drug-bearing nanoparticle delivery and microvascular ablation

우리는 permeabilization 및 / 또는 microvasculature의 절제가 초음파 pulsing 매개 변수를 변화에 의해 제어하는​​ 최소한 - 침입 대비 에이전트 microbubble 기반 치료 방법을 개발하고 있습니다. 특히, 우리는 이러한 접근법은 약물 전달 및 microvascular 절제를 통해 악성 뇌 종양을 치료하는 데 사용될 수 있는지 여부를 테스트하고 있습니다. 예비 연구 대상으로 약물 베어링 nanoparticle 배달 100nm 폴리 (lactide - 공동 glycolide) (PLAGA) 알부민 껍질 microbubbles을 준수 아르 nanoparticles로 구성된 &quot;합성&quot;배달 에이전트의 초음파 인한 파괴가 용이 될 수 있는지 여부를 결정하기 위해 수행되었습니다 . 우리는 microbubble - nanoparticle 합성 대리인 (MNCAs) 이러한 대리인을 나타냅니다. 초음파로 피하 C6 gliomas를 타겟으로하는 때, 우리는 microbubbles nanoparticles와 비 치료 종양에 비해 8.5 배 증가 공동 관리로 치료 종양여 MNCA 취급 종양에 nanoparticle 배달에 즉각 4.6 배 증가 관찰했다. 또한, 많은 암 애플 리케이션에서, 우리는 그것이 종양 hypoxia와 apoptosis으로 이어질 것입니다 종양 엑기스의 절제와 함께 타겟 약물 전달을 수행하는 것이 바람직하다 수 있습니다 믿습니다. 이를 위해, 우리는이 접근법은 종양 관류 감소, apoptosis, 상당한 성장 억제와 괴사를 elicits 것을​​ 보여주는 비 theramal 캐비테이션 유발 microvascular 박리의 효능을 테스트했습니다. 함께 촬영, 이러한 결과는 우리 초음파 타겟 접근 방식이 microvascular 절제 및 /를 통해 종양 괴사를 만들거나 동시에 gliomas에서 마약 페이로드를 강화하여 치료 효율을 높일 수있는 가능성이 있음을 나타냅니다.

Watch this Scientific Journal Video about Contrast Ultrasound Targeted Treatment of Gliomas in Mice via Drug-Bearing Nanoparticle Delivery and Microvascular Ablation at JoVE.com

Contrast Ultrasound Targeted Treatment of Gliomas in Mice via Drug-Bearing Nanoparticle Delivery and Microvascular Ablation

microbubbles의 Insonation는 유망 감소 시간 평균 음향 파워에서 종양 절제의 전략뿐만 아니라 치료제의 타겟으로 전달됩니다. 현재 연구의 목적은 피하 C6 gliomas가 아닌 열 microvascular 박리 및 페이로드 전송을 극대화하기 위해 낮은 듀티 사이클 초음파 pulsing 전략과 nanocarriers을 개발하는 것입니다.

명암 초음파 마약 베어링 Nanoparticle 배달 및 Microvascular 절제를 통해 마우스에서 Gliomas의 치료 타겟

We are developing minimally-invasive contrast agent microbubble based therapeutic approaches in which the permeabilization and/or ablation of the ...

Research

JoVE Journal

Bioengineering

Introduction to the Ultrasound Targeted Microbubble Destruction Technique

초음파 타겟 Microbubble 파괴 기술 소개

In UTMD, bioactive molecules, such as negatively charged plasmid DNA vectors encoding a gene of interest, are added to the cationic shells of lipid ...

연구

생체공학

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

타겟 안정 동위 원소 희석 액체 크로마 토그래피 - 질량 분광법 분석을위한 세포 지질 추출

The metabolism of fatty acids, such as arachidonic acid (AA) and linoleic acid (LA), results in the formation of oxidized bioactive lipids, including ...

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

표피 성장 인자를 사용하여 인간의 췌장 암 세포의 유전자 전달 및 Transfection은 다행 기반 엔지니어링된 Nanovectors를 수용체 타겟

More than 32,000 patients are diagnosed with pancreatic cancer in the United States per year and the disease is associated with very high mortality 1. ...

Evaluation of Polymeric Gene Delivery Nanoparticles by Nanoparticle Tracking Analysis and High-throughput Flow Cytometry

고분자 유전자 배달에 대한 평가는 Nanoparticle 추적 분석 및 높은 처리량 유동 세포 계측법에 의해 나노 입자

Non-viral gene delivery using polymeric nanoparticles has emerged as an attractive approach for gene therapy to treat genetic diseases1 and as a ...

Quantification of Global Diastolic Function by Kinematic Modeling-based Analysis of Transmitral Flow via the Parametrized Diastolic Filling Formalism

매개 변수화 이완기 충전 형식주의를 통해 Transmitral 흐름의 운동 학적 모델링 기반의 분석에 의하여 세계적인 이완기 기능의 정량화

Quantitative cardiac function assessment remains a challenge for physiologists and clinicians.&#160;Although historically invasive methods have comprised ...

Real-time Monitoring of High Intensity Focused Ultrasound (HIFU) Ablation of In Vitro Canine Livers Using Harmonic Motion Imaging for Focused Ultrasound (HMIFU)

Real-time Monitoring of High Intensity Focused Ultrasound HIFU Ablation of In Vitro Canine Livers Using Harmonic Motion Imaging for Focused Ultrasound HMIFU

높은 강도의 실시간 모니터링의 초음파 (HIFU) 절제를 집중<em&gt; 체외</em&gt; 집중 초음파에 대한 조화 운동 이미징을 사용하여 개 간을 (HMIFU)

Harmonic Motion Imaging for Focused Ultrasound (HMIFU) is a technique that can perform and monitor high-intensity focused ultrasound (HIFU) ablation. An ...

Targeted Plasma Membrane Delivery of a Hydrophobic Cargo Encapsulated in a Liquid Crystal Nanoparticle Carrier

액정 나노 입자 캐리어의 소수성화물 캡슐화의 대상 플라즈마 멤브레인 배달

The controlled delivery of drug/imaging agents to cells is critical for the development of therapeutics and for the study of cellular signaling processes. ...

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods

3 보완 방법을 사용 하 여 태 반 대상 약물 전달의 안전과 효율성의 종합 평가

No effective treatments currently exist for placenta-associated pregnancy complications, and developing strategies for the targeted delivery of drugs to ...

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

세포가 없는 세균 추출물, 리포좀 및 에멀젼 전달을 사용하여 합성 세포를 생산하는 단백질 을 준비합니다.

The bottom-up assembly approach for construction of synthetic cells is an effective tool for isolating and investigating cellular processes in a cell ...

Patient-Specific Polyvinyl Alcohol Phantom Fabrication with Ultrasound and X-Ray Contrast for Brain Tumor Surgery Planning

뇌종양 수술 계획을 위한 초음파 및 엑스레이 대비를 가진 환자 특정 폴리비닐 알콜 팬텀 제조

Phantoms are essential tools for clinical training, surgical planning and the development of novel medical devices. However, it is challenging to create ...