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DOI :
10.3791/2147-v
•
9:36 min
November 15th, 2010
Chapters
0:00
Title
0:58
Introduction
1:56
Preparation of Neurons
3:38
Click-iT 5-ethynyl-2-deoxyuridine (EdU) Labeling
5:31
Tyramide Signal Amplification (TSA) of EdU Signal
7:09
Visualization of Mitochondrial DNA Replication as a Marker for Mitochondiral Biogenesis
8:37
Conclusion
我们制定了一个敏感的技术标签在单个细胞中新合成的线粒体DNA(mtDNA),为了研究线粒体DNA的生物合成。该技术结合了一个酪胺信号放大(TSA)协议EDU纳入可视化的神经元亚细胞车厢内线粒体DNA的复制。
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