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DOI :
10.3791/2164-v
•
12:03 min
February 24th, 2011
Chapters
0:05
Title
1:21
Introduction
1:58
Preparation of Dialyzed Cell Lysate
4:48
Pouring of Blue Native-gels
7:04
Separation of Dialyzed Cell Lysate by Blue Native-PAGE
7:53
Second Dimension SDS-PAGE
9:25
Representative Results for Blue Native-PAGE Analysis of Multiprotein Complexes from Cellular Lysates
11:18
Conclusion
在这个视频中,我们描述的多蛋白复合物(储值卡)蓝色原生聚丙烯酰胺凝胶电泳(BN - PAGE)的表征。在第一个层面,透析细胞裂解液的BN - PAGE分离,以确定个人的多用途储值卡。在第二维SDS - PAGE电泳,利益的储值卡是进一步细分免疫印迹分析其成分。
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